Efficient <i>in vitro</i> multiplication of <i>Aechmea</i> 'Little Harv' and <i>Tillandsia cyanea</i> Linden ex K. Koch
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Date
2006
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Abstract
In vitro plant regeneration of Aechmea 'Little Harv' and Tillandsia cyanea, (Bromeliaceae), two endangered and important ornamental bromeliads native to Ecuador was achieved from seedling explants. T cyanea seedlings germinated or underwent indirect shoot organogenesis from callus when cultivated in a Murashige and Skoog (1962) nutrient medium in the presence of various levels of NAA (0.5 or 1.0 mg 1(-1)) in combination with BAP (0.1, 0.5 or 1.0 mg 1(-1)), plus 40 mg 1(-1) adenine. A. 'Little Harv' seed explants formed adventitious shoots without callus initiation. In both plants, the best shoot regeneration (approx. 9-10 shoots/explant) was obtained using 10 mg 1(-1) NAA and 0.1 mg 1(-1) BAP, in combination with adenine, but other growth regulator combinations were also successful. In the case of Aechmea, the addition of IAA instead of NAA was less efficient (2 shoots/explant). In addition to shoot formation 1 mg 1(-1) NAA and 0.1 mg 1(-1) induced callus on approx. 30% of T cyanea and 8% of A. 'Little Harv' explants. Isolated shoots formed roots after subculture in growth regulator-free media.
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adenine, endangered bromeliads, in vitro regeneration, ornamental bromeliads, shoot organogenesis