Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete <i>Ceriporiopsis subvermispora</i>
| dc.contributor.author | Tello, M | |
| dc.contributor.author | Seelenfreund, D | |
| dc.contributor.author | Lobos, S | |
| dc.contributor.author | Gaskell, J | |
| dc.contributor.author | Cullen, D | |
| dc.contributor.author | Vicuña, R | |
| dc.date.accessioned | 2025-01-21T01:30:51Z | |
| dc.date.available | 2025-01-21T01:30:51Z | |
| dc.date.issued | 2001 | |
| dc.description.abstract | Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of sport: forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB. their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of neu genes. (C) 2001 Federation of European Microbiological Societies. published by Elsevier Science B.V. All rights reserved. | |
| dc.fuente.origen | WOS | |
| dc.identifier.issn | 0378-1097 | |
| dc.identifier.uri | https://repositorio.uc.cl/handle/11534/96913 | |
| dc.identifier.wosid | WOS:000168888500015 | |
| dc.issue.numero | 1 | |
| dc.language.iso | en | |
| dc.pagina.final | 96 | |
| dc.pagina.inicio | 91 | |
| dc.revista | Fems microbiology letters | |
| dc.rights | acceso restringido | |
| dc.subject | homokaryon | |
| dc.subject | basidiomycete | |
| dc.subject | manganese peroxidase | |
| dc.subject | ligninolytic | |
| dc.subject | Ceriporiopsis subvermispora | |
| dc.subject.ods | 06 Clean Water and Sanitation | |
| dc.subject.odspa | 06 Agua limpia y saneamiento | |
| dc.title | Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete <i>Ceriporiopsis subvermispora</i> | |
| dc.type | artículo | |
| dc.volumen | 199 | |
| sipa.index | WOS | |
| sipa.trazabilidad | WOS;2025-01-12 |