Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in <i>Arabidopsis</i>

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dc.contributor.authorBlanco, F
dc.contributor.authorGarretón, V
dc.contributor.authorFrey, N
dc.contributor.authorDominguez, C
dc.contributor.authorPérez-Acle, T
dc.contributor.authorVan Der Straeten, D
dc.contributor.authorJordana, X
dc.contributor.authorHoluigue, L
dc.date.accessioned2025-01-21T01:06:37Z
dc.date.available2025-01-21T01:06:37Z
dc.date.issued2005
dc.description.abstractSalicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s11103-005-2227-x
dc.identifier.eissn1573-5028
dc.identifier.issn0167-4412
dc.identifier.urihttps://doi.org/10.1007/s11103-005-2227-x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96190
dc.identifier.wosidWOS:000233519700007
dc.issue.numero6
dc.language.isoen
dc.pagina.final944
dc.pagina.inicio927
dc.revistaPlant molecular biology
dc.rightsacceso restringido
dc.subjectas-1
dc.subjectcDNA-AFLP
dc.subjectcell protection to stress
dc.subjectearly activated genes
dc.subjectNPR1
dc.subjectsalicylic acid
dc.subject.ods02 Zero Hunger
dc.subject.ods13 Climate Action
dc.subject.odspa02 Hambre cero
dc.subject.odspa13 Acción por el clima
dc.titleIdentification of NPR1-dependent and independent genes early induced by salicylic acid treatment in <i>Arabidopsis</i>
dc.typeartículo
dc.volumen59
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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