A TAU FRAGMENT CONTAINING A REPETITIVE SEQUENCE INDUCES BUNDLING OF ACTIN-FILAMENTS

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dc.contributor.authorMORAGA, DM
dc.contributor.authorNUNEZ, P
dc.contributor.authorGARRIDO, J
dc.contributor.authorMACCIONI, RB
dc.date.accessioned2025-01-23T19:21:39Z
dc.date.available2025-01-23T19:21:39Z
dc.date.issued1993
dc.description.abstractMuch indirect evidence suggests that the interconnections of actin microfilaments with the microtubule system are mediated by microtubule-associated proteins (MAPs). In this study we provide new data to support the interaction of a specific tubulin-binding domain on tau with actin in vitro. In actin polymerization assays, the synthetic peptide VRSKIGSTENLKHQPGGG, corresponding to the first repetitive sequence Of tau protein, increased turbidity at 320 nm in a dose-dependent fashion. A salient feature of the tau peptide-induced assembly process is the formation of a large amount of actin filament bundles, as revealed by electron microscopic analysis. An increase in the tau peptide concentration resulted in a proportional increase in the bundling of actin filaments. It is interesting that a gradual decrease of pH within the range 7.6-4.7 resulted in a higher effect Of tau peptide in promoting bundles of actin filaments. A similar pH-dependent effect was observed for tau protein-induced bundling. An analysis of the mechanisms that operate in the peptide induction of actin filament bundles suggests the involvement of electrostatic forces, because the neutralization of epsilon-aminolysyl residues by selective carbamoylation resulted in a complete loss of the peptide induction of actin bundles. The data suggest that a tau repetitive sequence (also found in MAP-2 and MAP-4) containing a common tubulin binding motif may constitute a functional domain on tau for the dynamics of the interconnections between actin filaments and microtubules.
dc.fuente.origenWOS
dc.identifier.issn0022-3042
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98830
dc.identifier.wosidWOS:A1993LT33500023
dc.issue.numero3
dc.language.isoen
dc.pagina.final986
dc.pagina.inicio979
dc.revistaJournal of neurochemistry
dc.rightsacceso restringido
dc.subjectACTIN FILAMENTS
dc.subjectBUNDLING
dc.subjectMU PROTEINS
dc.subjectREPETITIVE SEQUENCES
dc.subjectSYNTHETIC PEPTIDES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA TAU FRAGMENT CONTAINING A REPETITIVE SEQUENCE INDUCES BUNDLING OF ACTIN-FILAMENTS
dc.typeartículo
dc.volumen61
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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