DISTRIBUTION OF INTERMEDIATE FILAMENTS IN AMPHIBIAN OXYNTIC CELLS - BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION

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dc.contributor.authorDABIKE, M
dc.contributor.authorKOENIG, CS
dc.contributor.authorVIAL, JD
dc.date.accessioned2025-01-23T19:44:12Z
dc.date.available2025-01-23T19:44:12Z
dc.date.issued1981
dc.description.abstractIntermediate filaments of toad oxyntic cells were isolated and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The major proteins of the residue were identified as actin and a 51,000 dalton polypeptide. Immunological crossreactivity between toad oxyntic cell intermediate filament components and antiprekeratin was shown by double immunodiffusion tests and indirect immunofluorescence. The immunofluorescent decoration of oxyntic cells and the EM images are coincident in locating the intermediate filaments mainly at the cortical and perinuclear basal zones. The cortical zone appears especially rich in prekeratin-like material at its adluminal 3rd. This results in a cup-like structure that encloses the cell portion occupied by the tubulovesicular system, which does not contain intermediate filaments. The translocation of membranes occurring during the secretory cycle of the oxyntic cell was attributed to a system of contractile proteins. The disposition of the prekeratin-like material suggests a role for intermediate filaments in the generation of movement produced by actin and myosin interaction, by providing a fixed plane for the anchoring of actin microfilaments.
dc.fuente.origenWOS
dc.identifier.eissn1432-0878
dc.identifier.issn0302-766X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99977
dc.identifier.wosidWOS:A1981MP97700004
dc.issue.numero4
dc.language.isoen
dc.pagina.final737
dc.pagina.inicio725
dc.revistaCell and tissue research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDISTRIBUTION OF INTERMEDIATE FILAMENTS IN AMPHIBIAN OXYNTIC CELLS - BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION
dc.typeartículo
dc.volumen220
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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