Peripheral binding site is involved in the neurotrophic activity of acetylcholinesterase

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dc.contributor.authorMuñoz, FJ
dc.contributor.authorAldunate, R
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:31:48Z
dc.date.available2025-01-21T01:31:48Z
dc.date.issued1999
dc.description.abstractACETYLCHOLINESTERASE (AChE) catalyses the hydrolysis of the neurotransmitter acetylcholine and it has been implicated in several non-cholinergic actions, including neurite outgrowth and amyloid formation. We have studied the trophic function of brain AChE on neuronal cell metabolism and proliferation as well as the enzyme domain involved in such effects. Low AChE concentrations (0.1-2.5 nM) stimulated neurite outgrowth and induced cell proliferation as measured by MTT reduction and [H-3]thymidine incorporation. The action of AChE was not affected by edrophonium and tacrine both active site inhibitors, but it was abolished by propidium and gallamine, two peripheral anionic binding site (PAS) ligands. We conclude that the PAS domain of AChE is involved in the neurotrophic activity of the enzyme. (C) 1999 Lippincott Williams & Wilkins.
dc.fuente.origenWOS
dc.identifier.eissn1473-558X
dc.identifier.issn0959-4965
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97146
dc.identifier.wosidWOS:000083934000030
dc.issue.numero17
dc.language.isoen
dc.pagina.final3625
dc.pagina.inicio3621
dc.revistaNeuroreport
dc.rightsacceso restringido
dc.subjectacetylcholinesterase
dc.subjectnon-cholinergic
dc.subjectneurotrophic activity
dc.subjectperipheral binding site
dc.subjectproliferation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePeripheral binding site is involved in the neurotrophic activity of acetylcholinesterase
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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