Heterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAs

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dc.contributor.authorFuentes, Yazmin
dc.contributor.authorOlguin, Valeria
dc.contributor.authorLopez-Ulloa, Brenda
dc.contributor.authorMendonca, Dafne
dc.contributor.authorRamos, Hade
dc.contributor.authorAbdalla, Ana Luiza
dc.contributor.authorGuajardo-Contreras, Gabriel
dc.contributor.authorNiu, Meijuan
dc.contributor.authorRojas-Araya, Barbara
dc.contributor.authorMouland, Andrew J.
dc.contributor.authorLopez-Lastra, Marcelo
dc.date.accessioned2025-01-20T17:10:48Z
dc.date.available2025-01-20T17:10:48Z
dc.date.issued2024
dc.description.abstractTranslation initiation of the human immunodeficiency virus-type 1 (HIV-1) genomic mRNA (vRNA) is cap-dependent or mediated by an internal ribosome entry site (IRES). The HIV-1 IRES requires IRES-transacting factors (ITAFs) for function. In this study, we evaluated the role of the heterogeneous nuclear ribonucleoprotein K (hnRNPK) as a potential ITAF for the HIV-1 IRES. In HIV-1-expressing cells, the depletion of hnRNPK reduced HIV-1 vRNA translation. Furthermore, both the depletion and overexpression of hnRNPK modulated HIV-1 IRES activity. Phosphorylations and protein arginine methyltransferase 1 (PRMT1)-induced asymmetrical dimethylation (aDMA) of hnRNPK strongly impacted the protein's ability to promote the activity of the HIV-1 IRES. We also show that hnRNPK acts as an ITAF for the human T cell lymphotropic virus-type 1 (HTLV-1) IRES, present in the 5 ' UTR of the viral sense mRNA, but not for the IRES present in the antisense spliced transcript encoding the HTLV-1 basic leucine zipper protein (sHBZ). This study provides evidence for a novel role of the host hnRNPK as an ITAF that stimulates IRES-mediated translation initiation for the retroviruses HIV-1 and HTLV-1.
dc.description.abstractGraphical Abstract
dc.fuente.origenWOS
dc.identifier.doi10.1093/nar/gkad1221
dc.identifier.eissn1362-4962
dc.identifier.issn0305-1048
dc.identifier.urihttps://doi.org/10.1093/nar/gkad1221
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91136
dc.identifier.wosidWOS:001134599700001
dc.issue.numero5
dc.language.isoen
dc.pagina.final2647
dc.pagina.inicio2625
dc.revistaNucleic acids research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHeterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAs
dc.typeartículo
dc.volumen52
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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