YEAST TRANSFER RNA3LEU GENE TRANSCRIBED AND SPLICED IN A HELA-CELL EXTRACT

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dc.contributor.authorSTANDRING, DN
dc.contributor.authorVENEGAS, A
dc.contributor.authorRUTTER, WJ
dc.date.accessioned2025-01-23T19:44:17Z
dc.date.available2025-01-23T19:44:17Z
dc.date.issued1981
dc.description.abstractA cloned yeast tRNA3Leu gene containing a 33-base intervening sequence (IVS) is selectively transcribed by a soluble extract from HeLa cells. The 130-nucleotide tRNA3Leu precursor RNA formed is colinear with the gene and contains approximately 4 leader nucleotides and up to 9 trailer nucleotides. The IVS is accurately and efficiently removed by an endogenous HeLa excision-ligase activity to yield the spliced tRNA, the free IVS, and the half-tRNA intermediates. The splicing reaction occurs without prior 5'' and 3'' maturation of the precursor but, with this exception, this pattern of synthesis and subsequent maturation of the tRNA3Leu precursor conforms to the scheme for tRNA biosynthesis deduced for the Xenopus system. The 3 systems utilize similar or identical tRNA3Leu precursors. Results stress the extraordinary conservation of tRNA biosynthesis in eukaryotes and demonstrate that a HeLa extract provides a useful system for investigating this process.
dc.fuente.origenWOS
dc.identifier.issn0027-8424
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99997
dc.identifier.wosidWOS:A1981MM06900003
dc.issue.numero10
dc.language.isoen
dc.pagina.final5967
dc.pagina.inicio5963
dc.revistaProceedings of the national academy of sciences of the united states of america-biological sciences
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleYEAST TRANSFER RNA3LEU GENE TRANSCRIBED AND SPLICED IN A HELA-CELL EXTRACT
dc.typeartículo
dc.volumen78
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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