Decreased caveolae in AGPAT2 lacking adipocytes is independent of changes in cholesterol or sphingolipid levels: A whole cell and plasma membrane lipidomic analysis of adipogenesis

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dc.article.number166167
dc.catalogadoraba
dc.contributor.authorGonzález-Hódar, L.
dc.contributor.authorMcDonald, J.G.
dc.contributor.authorVale, G.
dc.contributor.authorThompson, B.M.
dc.contributor.authorFigueroa, A.-M.
dc.contributor.authorTapia, P.J.
dc.contributor.authorRobledo, F.
dc.contributor.authorAgarwal, A.K.
dc.contributor.authorGarg, A.
dc.contributor.authorHorton, J.D.
dc.contributor.authorCortés, V.
dc.date.accessioned2024-03-04T15:19:41Z
dc.date.available2024-03-04T15:19:41Z
dc.date.issued2021
dc.description.abstractBackground Adipocytes from lipodystrophic Agpat2−/− mice have impaired adipogenesis and fewer caveolae. Herein, we examined whether these defects are associated with changes in lipid composition or abnormal levels of caveolae-associated proteins. Lipidome changes were quantified in differentiated Agpat2−/− adipocytes to identify lipids with potential adipogenic roles. Methods Agpat2−/− and wild type brown preadipocytes were differentiated in vitro. Plasma membrane was purified by ultracentrifugation. Number of caveolae and caveolae-associated proteins, as well as sterol, sphingolipid, and phospholipid lipidome were determined across differentiation. Results Differentiated Agpat2−/− adipocytes had decreased caveolae number but conserved insulin signaling. Caveolin-1 and cavin-1 levels were equivalent between Agpat2−/− and wild type adipocytes. No differences in PM cholesterol and sphingolipids abundance were detected between genotypes. Levels of phosphatidylserine at day 10 of differentiation were increased in Agpat2−/− adipocytes. Wild type adipocytes had increased whole cell triglyceride, diacylglycerol, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, and trihexosyl ceramide, and decreased 24,25-dihydrolanosterol and sitosterol, as a result of adipogenic differentiation. By contrast, adipogenesis did not modify whole cell neutral lipids but increased lysophosphatidylcholine, sphingomyelin, and trihexosyl ceramide levels in Agpat2−/− adipocytes. Unexpectedly, adipogenesis decreased PM levels of main phospholipids in both genotypes. Conclusion In Agpat2−/− adipocytes, decreased caveolae is not associated with changes in PM cholesterol nor sphingolipid levels; however, increased PM phosphatidylserine content may be implicated. Abnormal lipid composition is associated with the adipogenic abnormalities of Agpat2 −/− adipocytes but does not prevent insulin signaling.
dc.fechaingreso.objetodigital2024-12-17
dc.format.extent14 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.bbadis.2021.166167
dc.identifier.eissn1879-260X
dc.identifier.issn0925-4439
dc.identifier.urihttps://doi.org/10.1016/j.bbadis.2021.166167
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/82463
dc.identifier.wosidWOS:000659771300003
dc.information.autorucEscuela de Medicina; Cortes Mora, Victor Antonio; 0000-0002-1658-0965; 7576
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final14
dc.pagina.inicio1
dc.revistaBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
dc.rightsacceso restringido
dc.rights.licenseElsevier user license
dc.rights.urihttps://www.elsevier.com/about/policies-and-standards/open-access-licenses/user
dc.subjectAdipogenesis
dc.subjectCaveolae
dc.subjectLipids
dc.subjectPlasma membrane
dc.subjectagpat2
dc.subjectLipodystrophy
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDecreased caveolae in AGPAT2 lacking adipocytes is independent of changes in cholesterol or sphingolipid levels: A whole cell and plasma membrane lipidomic analysis of adipogenesis
dc.typeartículo
dc.volumen1867
sipa.codpersvinculados7576
sipa.trazabilidadORCID;2024-01-15
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