Clustering analyses of murine bone marrow-derived neutrophils reveal a phenotypic heterogeneity that can respond differentially to stimulation

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dc.article.numbere42227
dc.catalogadorpva
dc.contributor.authorSilva, Pedro H.
dc.contributor.authorPeñaloza Cerda, Hernán F.
dc.contributor.authorCordero, José
dc.contributor.authorKalergis Parra, Alexis Mikes
dc.contributor.authorBarrera Rojas, Nelson Patricio
dc.contributor.authorBueno Ramírez, Susan
dc.date.accessioned2025-03-10T20:39:19Z
dc.date.available2025-03-10T20:39:19Z
dc.date.issued2025
dc.description.abstractNeutrophils are granulocytic cells produced in the bone marrow from a granulocytic progenitor cell. During infection, the production of chemokines and cytokines induces the recruitment of neutrophils to the infected tissue to promote the clearance of microbial pathogens. Several studies have shown that different subpopulations of neutrophils can be identified during infection. However, no previous studies evaluated subpopulations of neutrophils purified from the bone marrow (BM), which are typically used to study the biology of these cells based on the assumption that the neutrophil population is homogeneous. In the present study, responses of purified BM-derived neutrophils to various stimuli such as PMA, LPS, and Streptococcus pneumoniae were evaluated using flow cytometry and bh-SNE analyses. Further, neutrophil population heterogeneity was assessed by clustering analyses. Our data suggest that purified BM-derived neutrophils were not a homogeneous cell population and were clustered into 12 subsets, each displaying a unique marker profile, where CD11b and CD62L emerged as pivotal markers for neutrophil function. Importantly, the subsets responded differentially to each stimulus, suggesting a nuanced activation pattern. Changes in biomarker expression were analyzed via Ingenuity Pathway Analysis (IPA) to unravel functional implications of the identified clusters, revealing subsets associated with different neutrophil functions, such as “Migration of neutrophils” or “Phagocytosis in neutrophils”. This study contributes to understanding the diversity of purified BM- derived neutrophils and the implications of using these cellular preparations to raise conclusions about the functionality of these cells in various infection models.
dc.fechaingreso.objetodigital2025-03-10
dc.format.extent9 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.heliyon.2025.e42227
dc.identifier.issn2405-8440
dc.identifier.urihttps://doi.org/10.1016/j.heliyon.2025.e42227
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102475
dc.information.autorucFacultad de Ciencias Biológicas; Silva, Pedro H.; S/I; 234277
dc.information.autorucFacultad de Ciencias Biológicas; Peñaloza Cerda, Hernán F.; S/I; 186994
dc.information.autorucFacultad de Ciencias Biológicas; Cordero, José; S/I; 197362
dc.information.autorucFacultad de Ciencias Biológicas; Kalergis Parra, Alexis Mikes; 0000-0001-7622-5263; 90610
dc.information.autorucFacultad de Ciencias Biológicas; Barrera Rojas, Nelson Patricio; 0000-0001-7825-1406; 4086
dc.information.autorucFacultad de Ciencias Biológicas; Bueno Ramírez, Susan; 0000-0002-7551-8088; 113541
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherElsevier Ltd.
dc.revistaHeliyon
dc.rightsacceso abierto
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleClustering analyses of murine bone marrow-derived neutrophils reveal a phenotypic heterogeneity that can respond differentially to stimulation
dc.typeartículo
dc.volumen11
sipa.codpersvinculados234277
sipa.codpersvinculados186994
sipa.codpersvinculados197362
sipa.codpersvinculados90610
sipa.codpersvinculados4086
sipa.codpersvinculados113541
sipa.trazabilidadORCID;2025-03-03
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