A novel pectin methylesterase inhibitor, PMEI3, in common bean suggests a key role of pectin methylesterification in Pseudomonas resistance

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dc.contributor.authorDe la Rubia, Alfonso G.
dc.contributor.authorLargo-Gosens, Asier
dc.contributor.authorYusta, Ricardo
dc.contributor.authorSepulveda-Orellana, Pablo
dc.contributor.authorRiveros, Anibal
dc.contributor.authorCenteno, Maria Luz
dc.contributor.authorSanhueza, Dayan
dc.contributor.authorMeneses, Claudio
dc.contributor.authorSaez-Aguayo, Susana
dc.contributor.authorGarcia-Angulo, Penelope
dc.date.accessioned2025-01-20T17:11:16Z
dc.date.available2025-01-20T17:11:16Z
dc.date.issued2024
dc.description.abstractThe mechanisms underlying susceptibility to and defense against Pseudomonas syringae (Pph) of the common bean (Phaseolus vulgaris) have not yet been clarified. To investigate these, 15-day-old plants of the variety Rinon were infected with Pph and the transcriptomic changes at 2 h and 9 h post-infection were analysed. RNA-seq analysis showed an up-regulation of genes involved in defense/signaling at 2 h, most of them being down-regulated at 9 h, suggesting that Pph inhibits the transcriptomic reprogramming of the plant. This trend was also observed in the modulation of 101 cell wall-related genes. Cell wall composition changes at early stages of Pph infection were associated with homogalacturonan methylation and the formation of egg boxes. Among the cell wall genes modulated, a pectin methylesterase inhibitor 3 (PvPMEI3) gene, closely related to AtPMEI3, was detected. PvPMEI3 protein was located in the apoplast and its pectin methylesterase inhibitory activity was demonstrated. PvPMEI3 seems to be a good candidate to play a key role in Pph infection, which was supported by analysis of an Arabidopsis pmei3 mutant, which showed susceptibility to Pph, in contrast to resistant Arabidopsis Col-0 plants. These results indicate a key role of the degree of pectin methylesterification in host resistance to Pph during the first steps of the attack.
dc.description.abstractSusceptibility of common bean to Pseudomonas infection results from changes in the degree of pectin methylesterification, in which a novel pectin methylesterase inhibitor, PMEI3, is involved.
dc.fuente.origenWOS
dc.identifier.doi10.1093/jxb/erad362
dc.identifier.eissn1460-2431
dc.identifier.issn0022-0957
dc.identifier.urihttps://doi.org/10.1093/jxb/erad362
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91170
dc.identifier.wosidWOS:001109624800001
dc.issue.numero1
dc.language.isoen
dc.pagina.final390
dc.pagina.inicio364
dc.revistaJournal of experimental botany
dc.rightsacceso restringido
dc.subjectCell wall
dc.subjecthalo blight disease
dc.subjecthomogalacturonan
dc.subjectpectin methylesterases
dc.subjectPhaseolus vulgaris
dc.subjectPseudomonas syringae
dc.titleA novel pectin methylesterase inhibitor, PMEI3, in common bean suggests a key role of pectin methylesterification in Pseudomonas resistance
dc.typeartículo
dc.volumen75
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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