Bioactivity of prolactin isoforms

Simple item page
dc.contributor.authorCampino, C
dc.contributor.authorTorres, C
dc.contributor.authorAmpuero, S
dc.contributor.authorDíaz, S
dc.contributor.authorGonzález, GB
dc.contributor.authorSerón-Ferré, M
dc.date.accessioned2025-01-21T01:32:08Z
dc.date.available2025-01-21T01:32:08Z
dc.date.issued1999
dc.description.abstractTo assess whether plasma prolactin (PRL) characteristics relate to lactogenesis and absence or presence of menstrual cycles, we measured bioactive PRL (BIO;PRL) using the Nb2 assay, immunoreactive PRL (TR-PRL) by radioimmunoassay, calculated equations describing the BIO-PRL-IR-PRL relationship and separated charged PIiL isoforms (by chromatofocusing) in five amenorrhoeic and five cycling nursing women at 6 months postpartum and in 10 cycling non-nursing women, Plasma samples were drawn before and 30 min after a suckling episode at 0800, 1600 and 2400 h in nursing women and at the same hours ih non-nursing women. BIO-PRL and IR-PRL concentrations were highest in amenorrhoeic nursing women, intermediate in cycling nursing women and lowest in cycling non-nursing women. The BIO-PRL-IR-PRL relationship shows that a given amount of IR-PRL corresponds to equivalent amounts of BIO-PRL in cycling nursing and cycling nonnursing women, and to a larger extent in amenorrhoeic nursing women. IR-PRL was present In plasma as several charge isoforms, Bioactive isoforms eluting at PH 6.0-5.1 were found in amenorrhoeic and cycling nursing women, reaching similar concentrations after suckling. Bioactive isoforms eluting at pH 7.0-6.1 were found only in amenorrhoeic nursing women. We speculate that isoforms eluting at pH 6.0-5.1 may play a role in lactation and isoforms eluting at pH 7.0-6.1, in lactational amenorrhoea.
dc.fuente.origenWOS
dc.identifier.issn0268-1161
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97210
dc.identifier.wosidWOS:000079783700009
dc.issue.numero4
dc.language.isoen
dc.pagina.final905
dc.pagina.inicio898
dc.revistaHuman reproduction
dc.rightsacceso restringido
dc.subjectlactation
dc.subjectlactational amenorrhoea
dc.subjectprolactin isoforms
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBioactivity of prolactin isoforms
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas