Seedborne <i>Cercospora beticola</i> Can Initiate Cercospora Leaf Spot from Sugar Beet (<i>Beta vulgaris</i>) Fruit Tissue

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dc.contributor.authorSpanner, Rebecca
dc.contributor.authorNeubauer, Jonathan
dc.contributor.authorHeick, Thies M.
dc.contributor.authorGrusak, Michael A.
dc.contributor.authorHamilton, Olivia
dc.contributor.authorRivera-Varas, Viviana
dc.contributor.authorde Jonge, Ronnie
dc.contributor.authorPethybridge, Sarah
dc.contributor.authorWebb, Kimberley M.
dc.contributor.authorLeubner-Metzger, Gerhard
dc.contributor.authorSecor, Gary A.
dc.contributor.authorBolton, Melvin D.
dc.date.accessioned2025-01-20T21:09:17Z
dc.date.available2025-01-20T21:09:17Z
dc.date.issued2022
dc.description.abstractCercospora leaf spot (CLS) is a globally important disease of sugar beet (Beta vulgaris) caused by the fungus Cercospora beticola. Long-distance movement of C. beticola has been indirectly evidenced in recent population genetic studies, suggesting potential dispersal via seed. Commercial sugar beet "seed" consists of the reproductive fruit (true seed surrounded by maternal pericarp tissue) coated in artificial pellet material. In this study, we confirmed the presence of viable C. beticola in sugar beet fruit for 10 of 37 tested seed lots. All isolates harbored the G143A mutation associated with quinone outside inhibitor resistance, and 32 of 38 isolates had reduced demethylation inhibitor sensitivity (EC50 > 1 mu g/ml). Planting of commercial sugar beet seed demonstrated the ability of seedborne inoculum to initiate CLS in sugar beet. C. beticola DNA was detected in DNA isolated from xylem sap, suggesting the vascular system is used to systemically colonize the host. We established nuclear ribosomal internal transcribed spacer region amplicon sequencing using the MinION platform to detect fungi in sugar beet fruit. Fungal sequences from 19 different genera were identified from 11 different sugar beet seed lots, but Fusarium, Alternaria, and Cercospora were consistently the three most dominant taxa, comprising an average of 93% relative read abundance over 11 seed lots. We also present evidence that C. beticola resides in the pericarp of sugar beet fruit rather than the true seed. The presence of seedborne inoculum should be considered when implementing integrated disease management strategies for CLS of sugar beet in the future.
dc.fuente.origenWOS
dc.identifier.doi10.1094/PHYTO-03-21-0113-R
dc.identifier.eissn1943-7684
dc.identifier.issn0031-949X
dc.identifier.urihttps://doi.org/10.1094/PHYTO-03-21-0113-R
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93522
dc.identifier.wosidWOS:000783100800001
dc.issue.numero5
dc.language.isoen
dc.pagina.final1028
dc.pagina.inicio1016
dc.revistaPhytopathology
dc.rightsacceso restringido
dc.subjectetiology
dc.subjectfungal pathogens
dc.subjectmicrobiome
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleSeedborne <i>Cercospora beticola</i> Can Initiate Cercospora Leaf Spot from Sugar Beet (<i>Beta vulgaris</i>) Fruit Tissue
dc.typeartículo
dc.volumen112
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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