Haem oxygenase 1 expression is altered in monocytes from patients with systemic lupus erythematosus

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dc.contributor.authorHerrada, Andres A.
dc.contributor.authorLlanos, Carolina
dc.contributor.authorMackern Oberti, Juan P.
dc.contributor.authorCarreno, Leandro J.
dc.contributor.authorHenriquez, Carla
dc.contributor.authorGomez, Roberto S.
dc.contributor.authorGutierrez, Miguel A.
dc.contributor.authorAnegon, Ignacio
dc.contributor.authorJacobelli, Sergio H.
dc.contributor.authorKalergis, Alexis M.
dc.date.accessioned2024-01-10T13:48:58Z
dc.date.available2024-01-10T13:48:58Z
dc.date.issued2012
dc.description.abstractSystemic lupus erythematosus (SLE) is an autoimmune disease characterized by multiple functional alterations affecting immune cells, such as B cells, T cells, dendritic cells (DCs) and monocytes. During SLE, the immunogenicity of monocytes and DCs is significantly up-regulated, promoting the activation of self-reactive T cells. Accordingly, it is important to understand the contribution of these cells to the pathogenesis of SLE and the mechanisms responsible for their altered functionality during disease. One of the key enzymes that control monocyte and DC function is haem oxygenase-1 (HO-1), which catalyses the degradation of the haem group into biliverdin, carbon monoxide and free iron. These products possess immunosuppressive and anti-inflammatory capacities. The main goal of this work was to determine HO-1 expression in monocytes and DCs from patients with SLE and healthy controls. Hence, peripheral blood mononuclear cells were obtained from 43 patients with SLE and 30 healthy controls. CD14+ monocytes and CD4+ T cells were sorted by FACS and HO-1 expression was measured by RT-PCR. In addition, HO-1 protein expression was determined by FACS. HO-1 levels in monocytes were significantly reduced in patients with SLE compared with healthy controls. These results were confirmed by flow cytometry. No differences were observed in other cell types, such as DCs or CD4+ T cells, although decreased MHC-II levels were observed in DCs from patients with SLE. In conclusion, we found a significant decrease in HO-1 expression, specifically in monocytes from patients with SLE, suggesting that an imbalance of monocyte function could be partly the result of a decrease in HO-1 expression.
dc.description.funderFONDECYT
dc.description.funderECOS-CONICYT
dc.description.funderBiomedical Research Consortium
dc.description.funderMillennium Institute on Immunology and Immunotherapy
dc.description.funderIMBIO , l'Agence de la Biomedecine, Ministere de la Recherche, Fondation CENTAURE, Fondation Progreffe
dc.fechaingreso.objetodigital2024-05-07
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1365-2567.2012.03598.x
dc.identifier.eissn1365-2567
dc.identifier.issn0019-2805
dc.identifier.pubmedidMEDLINE:22587389
dc.identifier.urihttps://doi.org/10.1111/j.1365-2567.2012.03598.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79411
dc.identifier.wosidWOS:000305893400007
dc.information.autorucCiencias Biológicas;Carreno L;S/I;120819
dc.information.autorucCiencias Biológicas;Gomez R;S/I;149736
dc.information.autorucMedicina;Gutierrez M;S/I;68377
dc.information.autorucMedicina;Henriquez C ;S/I;142421
dc.information.autorucCiencias Biológicas;Herrada A;S/I;174374
dc.information.autorucCiencias Biológicas;Kalergis A;S/I;90610
dc.information.autorucMedicina;Llanos C;S/I;1007976
dc.information.autorucCiencias Biológicas;Mackern J;S/I;1009722
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final424
dc.pagina.inicio414
dc.publisherWILEY
dc.revistaIMMUNOLOGY
dc.rightsacceso restringido
dc.subjecthaem oxygenase-1
dc.subjectmonocyte
dc.subjectsystemic lupus erythematosus
dc.subjectT-CELL
dc.subjectDENDRITIC CELLS
dc.subjectGENE PROMOTER
dc.subjectB-CELL
dc.subjectMICROSATELLITE POLYMORPHISM
dc.subjectMONOXIDE
dc.subjectHO-1
dc.subjectDISEASE
dc.subjectGAMMA
dc.subjectAUTOANTIBODIES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHaem oxygenase 1 expression is altered in monocytes from patients with systemic lupus erythematosus
dc.typeartículo
dc.volumen136
sipa.codpersvinculados120819
sipa.codpersvinculados149736
sipa.codpersvinculados68377
sipa.codpersvinculados142421
sipa.codpersvinculados174374
sipa.codpersvinculados90610
sipa.codpersvinculados1007976
sipa.codpersvinculados1009722
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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