Role of eukaryotic microbiota in soil survival and catabolic performance of the 2,4-D herbicide degrading bacteria Cupriavidus necator JMP134

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dc.contributor.authorManzano, Marlene
dc.contributor.authorMoran, Ana C.
dc.contributor.authorTesser, Bruno
dc.contributor.authorGonzalez, Bernardo
dc.date.accessioned2025-01-21T01:05:39Z
dc.date.available2025-01-21T01:05:39Z
dc.date.issued2007
dc.description.abstractCupriavidus necator ( formerly Ralstonia eutropha) JMP134, harbouring the catabolic plasmid pJP4, is the best-studied 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide degrading bacterium. A study of the survival and catabolic performance of strain JMP134 in agricultural soil microcosms exposed to high levels of 2,4-D was carried out. When C. necator JMP134 was introduced into soil microcosms, the rate of 2,4- D removal increased only slightly. This correlated with the poor survival of the strain, as judged by 16S rRNA gene terminal restriction fragment length polymorphism (T-RFLP) profiles, and the semi-quantitative detection of the pJP4-borne tfdA gene sequence, encoding the first step in 2,4- D degradation. After 3 days of incubation in irradiated soil microcosms, the survival of strain JMP134 dramatically improved and the herbicide was completely removed. The introduction of strain JMP134 into native soil microcosms did not produce detectable changes in the structure of the bacterial community, as judged by 16S rRNA gene T-RFLP profiles, but provoked a transient increase of signals putatively corresponding to protozoa, as indicated by 18S rRNA gene T-RFLP profiling. Accordingly, a ciliate able to feed on C. necator JMP134 could be isolated after soil enrichment. In native soil microcosms, C. necator JMP134 survived better than Escherichia coli DH5 alpha (pJP4) and similarly to Pseudomonas putida KT2442 ( pJP4), indicating that species specific factors control the survival of strains harbouring pJP4. The addition of cycloheximide to soil microcosms strongly improved survival of these three strains, indicating that the eukaryotic microbiota has a strong negative effect in bioaugmentation with catabolic bacteria.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s10482-006-9101-y
dc.identifier.eissn1572-9699
dc.identifier.issn0003-6072
dc.identifier.urihttps://doi.org/10.1007/s10482-006-9101-y
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95983
dc.identifier.wosidWOS:000244299800004
dc.issue.numeroNo. 2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final126
dc.pagina.inicio115
dc.revistaAntonie van Leeuwenhoek International Journal of General and Molecular Microbiology
dc.rightsacceso restringido
dc.subjectbioaugmentation
dc.subjectProtozoa
dc.subjectRalstonia eutropha JMP134
dc.subjecttfdA gene
dc.subjectT-RFLP
dc.titleRole of eukaryotic microbiota in soil survival and catabolic performance of the 2,4-D herbicide degrading bacteria Cupriavidus necator JMP134
dc.typeartículo
dc.volumenVol. 91
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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