Synthesis and processing of glypican during differentiation of skeletal muscle cells

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dc.catalogadoraba
dc.contributor.authorBrandan, Enrique
dc.contributor.authorCarey, D. J.
dc.contributor.authorLarraín Correa, Juan Agustín
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorCampos, A.
dc.date.accessioned2025-02-06T19:23:20Z
dc.date.available2025-02-06T19:23:20Z
dc.date.issued1996
dc.description.abstractWe identified previously a glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycan (HSPG) releasable by phosphatidylinositol-specific phospholipase C (PI-PLC) on the surface of differentiated skeletal muscle cells (Campos et al., Eur. J. Biochem. 216, 587-595 (1993)) which is homologous to the HSPG synthesized by fibroblasts and Schwann cells called glypican. In this study we have evaluated the processing, location and amount of this HSPG in skeletal muscle cells during differentiation. Immunoprecipitation of incubation medium obtained from differentiated cells incubated with [35S]sulfate by specific antibodies against glypican isolated from Schwann cells demonstrated that the antisera precipitated an intact HSPG. Immunoblot analysis of the proteins released by PI-PLC after heparitinase treatment revealed the presence of a main band of 64 and a faint band of 62 kDa, whereas the sizes of the core proteins for glypican present in the incubation media were 62 and 59 kDa. Pulse-chase experiments indicated that glypican present in the membrane was spontaneously released into the culture medium with a t1/2 of 12 h. The level of expression of glypican was analyzed during in vitro differentiation. The specific amount of the PI-PLC releasable HSPG increased about fourfold during cell differentiation. No changes were detected in the level of the mRNA for glypican. Indirect analysis revealed that in myotubes glypican is present on the cell surface as well as associated with the extracellular matrix (ECM). These results indicate that glypican is present, at least, in two different compartments on the surface of skeletal muscle cells.
dc.format.extent6 páginas
dc.fuente.origenSIPA
dc.identifier.eissn1618-1298
dc.identifier.issn0171-9335
dc.identifier.scopusid2-s2.0-0029860604
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102180
dc.identifier.wosidWOS:A1996VL74600006
dc.information.autorucFacultad de Ciencias Biológicas; Brandan, Enrique; 0000-0002-6820-5059; 52075
dc.information.autorucFacultad de Ciencias Biológicas; Larraín Correa, Juan Agustín; S/I; 90468
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.issue.numero2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final166
dc.pagina.inicio170
dc.revistaEuropean journal of cell biology
dc.rightsacceso restringido
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSynthesis and processing of glypican during differentiation of skeletal muscle cells
dc.typeartículo
dc.volumen71
sipa.codpersvinculados52075
sipa.codpersvinculados90468
sipa.codpersvinculados82342
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