Acidic pH and increasing [Ca(2+)] reduce the swelling of mucins in primary cultures of human cervical cells

Simple item page
dc.contributor.authorEspinosa, M.
dc.contributor.authorNoé, G.
dc.contributor.authorTroncoso, C.
dc.contributor.authorHo, S.B.
dc.contributor.authorVillalón, M.
dc.date.accessioned2025-01-21T01:10:38Z
dc.date.available2025-01-21T01:10:38Z
dc.date.issued2002
dc.description.abstractBACKGROUND: Cervical mucus is a heterogeneous mixture of water, ions and mucins that form a hydrophilic polymer gel. Mucins, the main components of mucus, are condensed inside secretory granules and swell to become a hydrogel after exocytosis. Using human cervical secretory cell primary cultures, the effect of [Ca2+] and [H+] on the swelling velocity of mucin granules was investigated in vitro. METHODS and RESULTS: Immunocytochemistry demonstrated that estrogen and progesterone receptors were expressed in cultured secretory cells along with mucins type 1, 4, 5AC and 5B. Exocytosis of secretory cells, recorded by videomicroscopy, showed that during swelling, the radius of the secretory granule matrix followed first-order kinetics. An increase in extracellular [Ca2+] from 1 to 4 mmol/l or a reduction in pH from 7.4 to 6.5 was seen to produce a significant decrease in the velocity of swelling of the secretory granule matrix. CONCLUSIONS: The inverse relationship observed between the diffusion of the granular matrix and the extracellular [Ca2+] or [H+] suggested that changes in cation concentration might drastically affect the swelling characteristics of mucins and provide a control mechanism for the observed viscoelastic properties of mucus.
dc.fechaingreso.objetodigital2025-02-01
dc.fuente.origenWOS
dc.identifier.issn0268-1161
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96704
dc.identifier.wosidWOS:000177228400009
dc.issue.numero8
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1972
dc.pagina.inicio1964
dc.revistaHuman reproduction
dc.rightsacceso restringido
dc.subjectcations
dc.subjectcervix
dc.subjectmucins
dc.subjectprimary cultures
dc.subjectswelling
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAcidic pH and increasing [Ca(2+)] reduce the swelling of mucins in primary cultures of human cervical cells
dc.typeartículo
dc.volumen17
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Acidic pH and increasing [Ca(2+)] reduce the swelling of mucins in primary cultures of human cervical cells.pdf
Size:
189.42 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas