Enrichment of electrochemically active microorganisms in a chlorate-pressured environment

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dc.catalogadorjlo
dc.contributor.authorCantillo González, Angela Patricia
dc.contributor.authorTorres Rojas, Felipe Ernesto
dc.contributor.authorDe La Iglesia Cabezas, Rodrigo Alonso
dc.contributor.authorVargas Cucurella, Ignacio Tomás
dc.date.accessioned2025-05-12T14:20:07Z
dc.date.available2025-05-12T14:20:07Z
dc.date.issued2025
dc.description.abstractThe study of bioelectrochemical systems (BESs) is gaining importance due to their versatility and wide applications, contributing to a circular bioeconomy. Electrochemically active microorganisms (EAMs) are crucial for catalyzing BES reactions and efficient operation. Applying poised potential in BESs has demonstrated the enrichment of EAMs and improved BES performance. However, in contaminated environments, it remains unclear whether the chemistry of the environment overshadows electrochemical selection. Chlorate is a toxic compound that restricts microbial community composition in BES. The aim of this study was to investigate the dynamic of electrochemical selection of electroactive chlorate-resistant microorganisms in a BES inoculated with sediment from northern Chile. For this purpose, sediment samples were enriched in a three-electrode cell through chronoamperometry with a poised potential of −0.55 V (vs. Ag/AgCl) for 112 days. Electrochemical activity was measured with cyclic voltammetry (CV), and bacterial growth was confirmed using scanning electron microscopy (SEM). Microbial diversity was analyzed via Next Generation Sequencing, and chlorate-resistance EAMs were isolated from the community obtained and characterized. The results showed the enrichment of EAMs that achieved a maximum cathodic current density of 21 μA/cm2. CV confirmed electrochemical activity, and microbial community analysis and scanning electron microscopy showed that attached and planktonic communities changed progressively throughout the experiment. From the communities, three new chlorate-resistant and electroactive isolates were obtained. Thus, this study demonstrated the feasibility of enriching EAMs from chlorate-pressured environments and paved the way for expanding electrochemical selection as a strategy for electro-bioaugmentation and bioremediation.
dc.format.extent13 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.jece.2025.116696
dc.identifier.urihttp://dx.doi.org/10.1016/j.jece.2025.116696
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104128
dc.information.autorucEscuela de Ingeniería; Cantillo González, Angela Patricia; S/I; 1092011
dc.information.autorucEscuela de Ingeniería; Torres Rojas, Felipe Ernesto; S/I; 1031254
dc.information.autorucFacultad de Ciencias Biológicas; De La Iglesia Cabezas, Rodrigo Alonso; 0000-0002-2000-8697; 13915
dc.information.autorucEscuela de Ingeniería; Vargas Cucurella, Ignacio Tomás; 0000-0001-5974-2795; 17080
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.revistaJournal of Environmental Chemical Engineering
dc.rightsacceso restringido
dc.subjectBioelectrochemical systems
dc.subjectChlorate
dc.subjectElectrochemical enrichment
dc.subject.ddcElectrochemically active microorganisms
dc.subject.ddc600
dc.subject.deweyCiencias de la computaciónes_ES
dc.titleEnrichment of electrochemically active microorganisms in a chlorate-pressured environment
dc.typeartículo
dc.volumen13
sipa.codpersvinculados1092011
sipa.codpersvinculados1031254
sipa.codpersvinculados13915
sipa.codpersvinculados17080
sipa.trazabilidadORCID;2025-05-07
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