Secretion of endoxylanase A from <i>Penicillium purpurogenum</i> by <i>Saccharomyces cerevisiae</i> transformed with genomic fungal DNA

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dc.contributor.authorChávez, R
dc.contributor.authorNavarro, C
dc.contributor.authorCalderón, I
dc.contributor.authorPeirano, A
dc.contributor.authorBull, P
dc.contributor.authorEyzaguirre, J
dc.date.accessioned2025-01-21T01:30:22Z
dc.date.available2025-01-21T01:30:22Z
dc.date.issued2002
dc.description.abstractSaccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.eissn1574-6968
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96723
dc.identifier.wosidWOS:000176884700015
dc.issue.numero2
dc.language.isoen
dc.pagina.final241
dc.pagina.inicio237
dc.revistaFems microbiology letters
dc.rightsacceso restringido
dc.subjectxylanases
dc.subjectheterologous gene expression
dc.subjectPenicillium purpurogenum
dc.subjectSaccharomyces cerevisiae
dc.subject.ods12 Responsible Consumption and Production
dc.subject.ods07 Affordable and Clean Energy
dc.subject.odspa12 Producción y consumo responsable
dc.subject.odspa07 Energía asequible y no contaminante
dc.titleSecretion of endoxylanase A from <i>Penicillium purpurogenum</i> by <i>Saccharomyces cerevisiae</i> transformed with genomic fungal DNA
dc.typeartículo
dc.volumen212
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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