Osteoclastogenesis in Patients With Systemic Sclerosis With and Without Calcinosis Cutis

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dc.article.numbere70029
dc.catalogadorpva
dc.contributor.authorValenzuela Vergara, Antonia María
dc.contributor.authorPérez, Guillermo
dc.contributor.authorChung, Lorinda
dc.contributor.authorSánchez, Felipe
dc.contributor.authorIturriaga Ortiz, Carolina Alejandra
dc.contributor.authorMontalva, Rebeca
dc.contributor.authorBorzutzky Schachter, Arturo
dc.date.accessioned2025-04-29T21:59:05Z
dc.date.available2025-04-29T21:59:05Z
dc.date.issued2025
dc.description.abstractObjective. We aimed to assess whether the presence of radiographically confirmed calcinosis of the hands in patients with systemic sclerosis (SSc) is associated with increased osteoclastogenesis. Methods. We recruited 20 patients with SSc (10 with calcinosis and 10 without calcinosis) and 10 age- and gender-matched healthy controls. Hand radiographs were scored using the validated Scleroderma Clinical Trials Consortium (SCTC) radiographic severity score for calcinosis. To evaluate osteoclastogenesis, peripheral blood mononuclear cells (PBMCs) were cultured with RANKL and macrophage colony-stimulating factor; osteoclasts were identified using tartrate-resistant acid phosphatase staining. Measures of bone resorption (RANKL, osteoprotegerin [OPG]) and ischemia or endothelial function (vascular endothelial growth factor, angiopoietin-1, and angiopoietin-2 [Ang-2]) were also evaluated. Results. Patients with SSc were all women and Hispanic, and the majority (n = 12, 60%) had limited SSc skin type. Mean +/- SD age was 55.2 +/- 14.8 years; mean +/- SD disease duration was 9.5 +/- 6.5 years from first non-Raynaud phenomenon symptom. Patients with SSc with calcinosis had more digital ischemia than patients without calcinosis. Median SCTC score in patients with SSc with calcinosis was 11.1 (range 0.7-286). After 9 days in culture, PBMCs from patients with calcinosis originated a significantly higher number of osteoclasts (33.0 +/- 20.3 cells/well) than patients without calcinosis (15.3 +/- 6.9 cells/well) and healthy individuals (11.2 +/- 2.6 cells/well) (P = 0.001). The severity of calcinosis was not correlated with the number of osteoclasts per well (r = 0.27, P = 0.5); however, it was correlated with RANKL (r = 0.82, P = 0.004), RANKL/OPG ratio (r = 0.86, P = 0.002), and Ang-2 levels (r = 0.86, P = 0.002). Conclusion. Calcinosis in patients with SSc is associated with an increased propensity of peripheral blood cells to form osteoclasts. Targeted inhibition of osteoclastogenesis may provide a specific therapeutic option for patients with SSc-associated calcinosis.
dc.description.funderScleroderma Clinical Trials Consortium
dc.fechaingreso.objetodigital2025-04-29
dc.format.extent7 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1002/acr2.70029
dc.identifier.issn2578-5745
dc.identifier.urihttps://doi.org/10.1002/acr2.70029
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/103540
dc.identifier.wosidWOS:001458339900001
dc.information.autorucEscuela de Medicina; Valenzuela Vergara, Antonia María; 0000-0003-3357-9402; 1082691
dc.information.autorucEscuela de Medicina; Iturriaga Ortiz, Carolina Alejandra; S/I; 1058623
dc.information.autorucEscuela de Medicina; Borzutzky Schachter, Arturo; 0000-0002-7904-262X; 5897
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherWiley
dc.revistaACR Open Rheumatology
dc.rightsacceso abierto
dc.rights.licenseAttribution-NonCommercial 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleOsteoclastogenesis in Patients With Systemic Sclerosis With and Without Calcinosis Cutis
dc.typeartículo
dc.volumen7
sipa.codpersvinculados1082691
sipa.codpersvinculados1058623
sipa.codpersvinculados5897
sipa.trazabilidadORCID;2025-04-21
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