Evaluation of direct immunofluorescent assay (DFA) and rapid antigen test (RAT) for diagnosis of new pandemic influenza A H1N1 2009 (FLU AH1N1) during first wave in Santiago, Chile

Simple item page
dc.catalogadorjca
dc.contributor.authorVizcaya, C.
dc.contributor.authorFerres, M.
dc.contributor.authorPerret Pérez, Cecilia
dc.contributor.authorMartinez, C.
dc.contributor.authorGodoy, P.
dc.contributor.authorContreras, A. M.
dc.contributor.authorFerrer, P.
dc.contributor.authorAzocar, T.
dc.date.accessioned2025-03-11T13:34:41Z
dc.date.available2025-03-11T13:34:41Z
dc.date.issued2010
dc.description.abstractBackground: Since May 17th 2009 (epidemiological week 20th), the new strain of influenza A H1N1 was detected in respiratory samples of symptomatic patients in Santiago, Chile. The circulation of the virus lasted 11 weeks, with a peak between weeks 25-27th. The objective of our study was to evaluate the performance of influenza tests for diagnosis of FLU AH1N1. Methods: Nasopharyngeal swabs were taken from in and outpatients with influenza like illness (ILI), between June 1st and July 19th of 2009 (weeks 23-29th) and the results of DFA and RAT were compared using RT-PCR FLU AH1N1 (Light mix Kit Influenza A virus M2 and Light Mix Kit FLU A swine H1Ò of TIB MOLBIOL) as gold standard. We analyzed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of DFA (D3 Ultra 8 DFA Respiratory & Identification Kit ä de Diagnostic Hybrid) and RAT (QuickVeuÒ of Biomerieux). Results were compared by age group and over three different periods of the outbreak: increasing, peak and decreasing. Results: 510 patients had RT-PCR for FLU AH1N1 with simultaneous DFA, 385 with RAT and 48 with both tests. Average age with DFA was 25,8 years (1 month-108 years, 53% females) and with RAT 32,9 years (2 months-108 years, 51% females), (p <0,0001). Comparing periods of the outbreak, DFA sensitivity was 58%, 77% and 81% in ascending, peak and descending period, respectively (p <0,001) and specificity was 90%, 83% and 91% respectively (p>0,05). Evaluating RAT, sensitivity was 41%, 61% and 67% (p<0,001) and specificity was 87%, 96% y 92% (p> 0,05) in different periods.
dc.format.extent2 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.ijid.2010.02.1709
dc.identifier.issn1201-9712
dc.identifier.urihttps://doi.org/10.1016/j.ijid.2010.02.1709
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102482
dc.identifier.wosidWOS:000276298200230
dc.information.autorucEscuela de Medicina; Perret Pérez, Cecilia; 0000-0002-1535-1204; 80387
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.finalE101
dc.pagina.inicioE100
dc.publisherELSEVIER SCI LTD
dc.rightsacceso restringido
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEvaluation of direct immunofluorescent assay (DFA) and rapid antigen test (RAT) for diagnosis of new pandemic influenza A H1N1 2009 (FLU AH1N1) during first wave in Santiago, Chile
dc.typecomunicación de congreso
dc.volumen14
sipa.codpersvinculados80387
Files
Collections
Artículos de conferencia