ACTIN AND MYOSIN IN OXYNTIC CELL - GELATION AND CONTRACTION OF CRUDE EXTRACTS INVITRO
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Date
1981
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Abstract
Extracts prepared from purified toad [Bufo spinulosus] oxyntic cells undergo temperature-dependent, cytochalasin B (CB)-sensitive gelation. In the presence of ATP, the interaction between gelled actin and myosin produces a contracted gel. Such association ceases spontaneously after shrinkage is completed. A stable myosin-actin interaction takes place in the absence of ATP, but no contraction of the gel is observed. A correlation between this actomyosin system present in oxyntic cells and the high mobility of the secretory pole, resulting in the changes in shape observed during the onset of HCl secretion, is proposed.