The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

Simple item page
dc.contributor.authorLoyola, Rodrigo
dc.contributor.authorHerrera, Daniela
dc.contributor.authorMas, Abraham
dc.contributor.authorWong, Darren Chern Jan
dc.contributor.authorHoell, Janine
dc.contributor.authorCavallini, Erika
dc.contributor.authorAmato, Alessandra
dc.contributor.authorAzuma, Akifumi
dc.contributor.authorZiegler, Tobias
dc.contributor.authorAquea, Felipe
dc.contributor.authorCastellarin, Simone Diego
dc.contributor.authorBogs, Jochen
dc.contributor.authorTornielli, Giovanni Battista
dc.contributor.authorPena-Neira, Alvaro
dc.contributor.authorCzemmel, Stefan
dc.contributor.authorAntonio Alcalde, Jose
dc.contributor.authorTomas Matus, Jose
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-23T21:28:56Z
dc.date.available2025-01-23T21:28:56Z
dc.date.issued2016
dc.description.abstractBy performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis.Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera. We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.
dc.fuente.origenWOS
dc.identifier.doi10.1093/jxb/erw307
dc.identifier.eissn1460-2431
dc.identifier.issn0022-0957
dc.identifier.urihttps://doi.org/10.1093/jxb/erw307
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101418
dc.identifier.wosidWOS:000386067000014
dc.issue.numero18
dc.language.isoen
dc.pagina.final5445
dc.pagina.inicio5429
dc.revistaJournal of experimental botany
dc.rightsacceso restringido
dc.subjectBinding
dc.subjectglycosyltransferase
dc.subjectMYBF1
dc.subjectnetwork
dc.subjectphotolyase
dc.subjectripening
dc.subjectUVR8
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleThe photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment
dc.typeartículo
dc.volumen67
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas