Differential gene expression in <i>Pyropia columbina</i> (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

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dc.contributor.authorContreras-Porcia, Loretto
dc.contributor.authorLopez-Cristoffanini, Camilo
dc.contributor.authorLovazzano, Carlos
dc.contributor.authorRosa Flores-Molina, Maria
dc.contributor.authorThomas, Daniela
dc.contributor.authorNunez, Alejandra
dc.contributor.authorFierro, Camila
dc.contributor.authorGuajardo, Eduardo
dc.contributor.authorCorrea, Juan A.
dc.contributor.authorKube, Michael
dc.contributor.authorReinhardt, Richard
dc.date.accessioned2025-01-24T00:07:18Z
dc.date.available2025-01-24T00:07:18Z
dc.date.issued2013
dc.description.abstractIn rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/ proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.
dc.fuente.origenWOS
dc.identifier.doi10.3856/vol41-issue5-fulltext-13
dc.identifier.eissn0717-7178
dc.identifier.issn0718-560X
dc.identifier.urihttps://doi.org/10.3856/vol41-issue5-fulltext-13
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101910
dc.identifier.wosidWOS:000328337900013
dc.issue.numero5
dc.language.isoen
dc.pagina.final958
dc.pagina.inicio933
dc.revistaLatin american journal of aquatic research
dc.rightsacceso restringido
dc.subjectPyropia
dc.subjectdesiccation stress
dc.subjectESTs
dc.subjectseaweeds
dc.subjecttranscriptomics
dc.subjectproteins
dc.subject.ods14 Life Below Water
dc.subject.ods13 Climate Action
dc.subject.ods15 Life on Land
dc.subject.odspa14 Vida submarina
dc.subject.odspa13 Acción por el clima
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleDifferential gene expression in <i>Pyropia columbina</i> (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
dc.typeartículo
dc.volumen41
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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