Transport and Secretion of the Wnt3 Ligand by Motor Neuron-like Cells and Developing Motor Neurons

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dc.contributor.authorPinto, Cristina
dc.contributor.authorPerez, Viviana
dc.contributor.authorMella, Jessica
dc.contributor.authorAlbistur, Miguel
dc.contributor.authorCaprile, Teresa
dc.contributor.authorBronfman, Francisca C.
dc.contributor.authorHenriquez, Juan Pablo
dc.date.accessioned2025-01-20T22:03:24Z
dc.date.available2025-01-20T22:03:24Z
dc.date.issued2021
dc.description.abstractThe vertebrate neuromuscular junction (NMJ) is formed by a presynaptic motor nerve terminal and a postsynaptic muscle specialization. Cumulative evidence reveals that Wnt ligands secreted by the nerve terminal control crucial steps of NMJ synaptogenesis. For instance, the Wnt3 ligand is expressed by motor neurons at the time of NMJ formation and induces postsynaptic differentiation in recently formed muscle fibers. However, the behavior of presynaptic-derived Wnt ligands at the vertebrate NMJ has not been deeply analyzed. Here, we conducted overexpression experiments to study the expression, distribution, secretion, and function of Wnt3 by transfection of the motor neuron-like NSC-34 cell line and by in ovo electroporation of chick motor neurons. Our findings reveal that Wnt3 is transported along motor axons in vivo following a vesicular-like pattern and reaches the NMJ area. In vitro, we found that endogenous Wnt3 expression increases as the differentiation of NSC-34 cells proceeds. Although NSC-34 cells overexpressing Wnt3 do not modify their morphological differentiation towards a neuronal phenotype, they effectively induce acetylcholine receptor clustering on co-cultured myotubes. These findings support the notion that presynaptic Wnt3 is transported and secreted by motor neurons to induce postsynaptic differentiation in nascent NMJs.
dc.fuente.origenWOS
dc.identifier.doi10.3390/biom11121898
dc.identifier.eissn2218-273X
dc.identifier.urihttps://doi.org/10.3390/biom11121898
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94038
dc.identifier.wosidWOS:000736208300001
dc.issue.numero12
dc.language.isoen
dc.revistaBiomolecules
dc.rightsacceso restringido
dc.subjectWnt
dc.subjectmotor neuron
dc.subjectneuromuscular junction
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTransport and Secretion of the Wnt3 Ligand by Motor Neuron-like Cells and Developing Motor Neurons
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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