UL146 variability among clinical isolates of Human Cytomegalovirus from Japan

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dc.contributor.authorAguayo, Francisco
dc.contributor.authorMurayama, Tsugiya
dc.contributor.authorEizuru, Yoshito
dc.date.accessioned2024-01-10T14:22:20Z
dc.date.available2024-01-10T14:22:20Z
dc.date.issued2010
dc.description.abstractHuman Cytomegalovirus (HCMV) is a herpesvirus associated with serious diseases in immunocompromised subjects. The region between ORF UL133 and UL151 from HCMV, named ULb' is frequently deleted in attenuated AD169 and in highly passaged laboratory strains. However, this region is conserved in low-passaged and more virulent HCMV, like the Toledo strain. The UL146 gene, which is located in the ULb' region, encodes a CXC-chemokine analogue. The diversity of UL146 gene was evaluated among fifty-six clinical isolates of HCMV from Japan. Results show that UL146 gene was successfully amplified by the polymerase chain reaction (PCR) in only 17/56 strains (30%), while the success rate for UL145/UL147 gene was 18/56 strains (32%). After DNA sequencing, the 35 amplified strains were classified into 8 groups. When compared, variability of UL146 ranged from 25.1% to 52.9% at the DNA level and from 34.5% to 67% at the amino acid level. Seven groups had the interleukin-8 (IL-8) motif ERL (Glu-Leu-Arg) CXC and one group had only the CXC motif, suggesting the absence of the IL-8 function of UL146. In conclusion, we found that UL146 gene of HCMV is hypervariable in clinical strains from Japan suggesting the possibility of a different function in each sequence group.
dc.description.funderMinistry of Education, Culture, Sports, Science and Technology, Japan
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.4067/S0716-97602010000400013 
dc.identifier.issn0716-9760
dc.identifier.pubmedidMEDLINE:21526275
dc.identifier.uri10.4067/S0716-97602010000400013 
dc.identifier.wosidWOS:000286413300013
dc.information.autorucMedicina;Aguayo F ;S/I;1005475
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final480
dc.pagina.inicio475
dc.publisherSOC BIOLGIA CHILE
dc.revistaBIOLOGICAL RESEARCH
dc.rightsacceso abierto
dc.subjectCytomegalovirus
dc.subjectUL146
dc.subjectSequence
dc.subjectV
dc.subjectOPEN READING FRAME
dc.subjectSEQUENCE VARIABILITY
dc.subjectALPHA-CHEMOKINE
dc.subjectSTRAINS
dc.subjectGENES
dc.subjectANGIOGENESIS
dc.subjectRECIPIENTS
dc.subjectGENOTYPES
dc.subjectVIRUS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleUL146 variability among clinical isolates of Human Cytomegalovirus from Japan
dc.typeartículo
dc.volumen43
sipa.codpersvinculados1005475
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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