Acute lung injury secondary to hydrochloric acid instillation induces small airway hyperresponsiveness

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dc.catalogadordfo
dc.contributor.authorBasoalto Escobar, Roque Ignacio
dc.contributor.authorDamiani Rebolledo, Luis Felipe
dc.contributor.authorBachmann Barron, María Consuelo
dc.contributor.authorFonseca, Marcelo
dc.contributor.authorBarros, Marisol
dc.contributor.authorSoto Muñoz, Dagoberto Igor
dc.contributor.authorAraos, Joaquin
dc.contributor.authorJalil Contreras, Yorschua Frederick
dc.contributor.authorDubo, Sebastian
dc.contributor.authorRetamal, Jaime
dc.contributor.authorBugedo Tarraza, Guillermo Jaime
dc.contributor.authorHenriquez, Mauricio
dc.contributor.authorBruhn Cruz, Alejandro Rodrigo
dc.date.accessioned2024-10-22T13:43:51Z
dc.date.available2024-10-22T13:43:51Z
dc.date.issued2021
dc.description.abstractBackground: Acute respiratory distress syndrome (ARDS) is a severe form of respiratory failure characterized by altered lung mechanics and poor oxygenation. Bronchial hyperresponsiveness has been reported in ARDS survivors and animal models of acute lung injury. Whether this hyperreactivity occurs at the small airways or not is unknown. Objective: To determine ex-vivo small airway reactivity in a rat model of acute lung injury (ALI) by hydrochloric acid (HCl) instillation. Methods: Twelve anesthetized rats were connected to mechanical ventilation for 4-hour, and randomly allocated to either ALI group (HCl intratracheal instillation; n=6) or Sham (intratracheal instillation of 0.9% NaCl; n=6). Oxygenation was assessed by arterial blood gases. After euthanasia, tissue samples from the right lung were harvested for histologic analysis and wet-dry weight ratio assessment. Precision cut lung slice technique (100-200 pm diameter) was applied in the left lung to evaluate ex vivo small airway constriction in response to histamine and carbachol stimulation, using phase-contrast video microscopy. Results: Rats from the ALI group exhibited hypoxemia, worse histologic lung injury, and increased lung wet-dry weight ratio as compared with the sham group. The bronchoconstrictor responsiveness was significantly higher in the ALI group, both for carbachol (maximal contraction of 84.5 +/- 2.5% versus 61.4 +/- 4.2% in the Sham group, P<0.05), and for histamine (maximal contraction of 78.6 +/- 5.3% versus 49.6 +/- 5.3% in the Sham group, P<0.05). Conclusion: In an animal model of acute lung injury secondary to HCL instillation, small airway hyperresponsiveness to carbachol and histamine is present. These results may provide further insight into the pathophysiologi of ARDS.
dc.description.funderCONICYT
dc.description.funderFONDECYT
dc.description.funderCONICYT-PFCHA/Doctorado Nacional 2020-folio
dc.description.funderCONICYT-PFCHA/Doctorado Nacional 2019-folio
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.issn1943-8141
dc.identifier.pubmedidMEDLINE:34956488
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/88316
dc.identifier.wosidWOS:000740433300004
dc.information.autorucEscuela de Medicina; Basoalto Escobar Roque Ignacio; 0000-0002-8908-7397; 1010703
dc.information.autorucEscuela de Medicina; Damiani Rebolledo Luis Felipe; 0000-0002-8338-0488; 237645
dc.information.autorucEscuela de Medicina; Bachmann Barron Maria Consuelo; 0000-0002-2006-0591; 215825
dc.information.autorucEscuela de Medicina; Soto Munoz Dagoberto Igor; 0000-0002-7350-1530; 1008458
dc.information.autorucEscuela de Medicina; Jalil Contreras Yorschua Frederick; 0000-0002-4993-7158; 1079709
dc.information.autorucEscuela de Medicina; Bugedo Tarraza Guillermo Jaime; 0000-0001-7527-6202; 60490
dc.information.autorucEscuela de Medicina; Bruhn Cruz Alejandro Rodrigo; S/I; 741
dc.issue.numero11
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final12741
dc.pagina.inicio12734
dc.revistaAmerican Journal of Translational Research
dc.rightsregistro bibliográfico
dc.subjectARDS (acute respiratory distress syndrome)
dc.subjectALI (acute lung injury)
dc.subjectSmall airway hyperresponsiveness
dc.subjectAcid aspiration
dc.subjectExpiratory flow limitation
dc.subjectRespiratory-distress-syndrome
dc.subjectGas-exchange
dc.subjectSlices PCLS
dc.subjectPressure
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAcute lung injury secondary to hydrochloric acid instillation induces small airway hyperresponsiveness
dc.typeartículo
dc.volumen13
sipa.codpersvinculados1010703
sipa.codpersvinculados237645
sipa.codpersvinculados215825
sipa.codpersvinculados1008458
sipa.codpersvinculados1079709
sipa.codpersvinculados60490
sipa.codpersvinculados741
sipa.trazabilidadWOS;18-03-2022
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