Lack of Norovirus Replication and Histo-Blood Group Antigen Expression in 3-Dimensional Intestinal Epithelial Cells

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dc.contributor.authorHerbst-Kralovetz, Melissa M.
dc.contributor.authorRadtke, Andrea L.
dc.contributor.authorLay, Margarita K.
dc.contributor.authorHjelm, Brooke E.
dc.contributor.authorBolick, Alice N.
dc.contributor.authorSarker, Shameema S.
dc.contributor.authorAtmar, Robert L.
dc.contributor.authorKingsley, David H.
dc.contributor.authorArntzen, Charles J.
dc.contributor.authorEstes, Mary K.
dc.contributor.authorNickerson, Cheryl A.
dc.date.accessioned2025-01-24T00:07:28Z
dc.date.available2025-01-24T00:07:28Z
dc.date.issued2013
dc.description.abstractNoroviruses (NoVs) are a leading cause of gastroenteritis worldwide. An in vitro model for NoV replication remains elusive, making study of the virus difficult. A previous study, which used a 3-dimensional (3-D) intestinal model derived from INT-407 cells reported NoV replication and extensive cytopathic effects (CPE). Using the same 3-D model, but with highly purified Norwalk virus (NV), we attempted to replicate this study. Our results showed no evidence of NV replication by real-time PCR of viral RNA or by immunocytochemical detection of viral structural and nonstructural proteins. Immunocytochemical analysis of the 3-D cultures also showed no detectable presence of histo-blood group antigens that participate in NV binding and host tropism.,To determine the potential cause of ORE observed in the previous study, we exposed 3-D cultures to lipopolysaccharide concentrations consistent with contaminated stool samples and observed morphologic features similar to CPE. We conclude that the 3-D INT-407 model does not support NV replication.
dc.fuente.origenWOS
dc.identifier.doi10.3201/eid1903.121029
dc.identifier.eissn1080-6059
dc.identifier.issn1080-6040
dc.identifier.urihttps://doi.org/10.3201/eid1903.121029
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101915
dc.identifier.wosidWOS:000328173000011
dc.issue.numero3
dc.language.isoen
dc.pagina.final438
dc.pagina.inicio431
dc.revistaEmerging infectious diseases
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLack of Norovirus Replication and Histo-Blood Group Antigen Expression in 3-Dimensional Intestinal Epithelial Cells
dc.typeartículo
dc.volumen19
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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