Biofilm formation on collagen substrates modulates Streptococcus mutans bacterial extracellular nanovesicle production and cargo

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dc.catalogadorvdr
dc.contributor.authorLeiva Sabadini, Camila Andrea
dc.contributor.authorBerríos Segovia, Pablo Germán
dc.contributor.authorSaavedra Godoy, Paula Andrea
dc.contributor.authorCarrasco Rojas, Javiera
dc.contributor.authorGonzález Aramundiz, José Vicente
dc.contributor.authorVera Véliz, Mario Andrés
dc.contributor.authorTarifeño Saldivia, Estefanía
dc.contributor.authorSchuh, Christina M. A. P.
dc.contributor.authorAguayo Paul, Sebastián
dc.date.accessioned2025-08-29T17:16:42Z
dc.date.available2025-08-29T17:16:42Z
dc.date.issued2025
dc.description.abstractStreptococcus mutans is the major microbial etiological agent of dental caries and can adhere to surfaces such as type-I collagen, which is present in dentin and periodontal tissues. Recent studies have characterized planktonic S. mutans bacterial extracellular vesicles (bEVs) at the nanoscale range and demonstrated environmental-induced changes due to sugar presence or pH alterations. However, to date, no studies have explored whether surface-derived changes can modulate bEV production in the context of oral biofilm formation in the elderly. Therefore, this work aimed to determine the role of biofilm formation and collagen glycation on the nanoscale morphology and proteomic composition of S. mutans bEVs. For this, bEVs from S. mutans biofilms on native and glycated collagen surfaces were isolated, characterized, and compared to bEVs from planktonic cells. Nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), and electron microscopy confirmed bEV production and showed that bEVs from biofilms are smaller in size and less abundant than those from planktonic cells. Furthermore, proteome analysis revealed that S. mutans biofilm formation on native and glycated collagen led to the enrichment of several key virulence proteins. Also, a shift towards proteins involved in metabolic processes was found in bEVs following biofilm formation on collagen surfaces, whereas glucan metabolism proteins were overexpressed in vesicles from the planktonic state. These results demonstrate that biofilm formation, as well as the glycation of collagen associated with aging and hyperglycaemia, can modulate bEV characteristics and cargo and could play a central role in S. mutans virulence and the development of diseases such as dental caries and periodontal disease.
dc.description.funderANID/FONDECYT; Folios: 1220804 y 1220803
dc.fechaingreso.objetodigital2025-08-28
dc.format.extent11 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1039/D5NA00248F
dc.identifier.urihttps://doi.org/10.1039/D5NA00248F
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105385
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Leiva Sabadini, Camila Andrea; S/I; 1027766
dc.information.autorucFacultad de Ciencias Biológicas; Berríos Segovia, Pablo Germán; S/I; 217256
dc.information.autorucFacultad de Ciencias Biológicas; Saavedra Godoy, Paula Andrea; S/I; 1028068
dc.information.autorucEscuela de Química; González Aramundiz, José Vicente; 0000-0001-7856-9211; 17164
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Vera Véliz, Mario Andrés; 0000-0002-1762-4421; 1024156
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Aguayo Paul, Sebastián; 0000-0003-0900-1993; 1062336
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final11
dc.pagina.inicio1
dc.revistaNanoscale Advances
dc.rightsacceso abierto
dc.rights.licenseCC BY-NC 3.0 Attribution-NonCommercial 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subject.ddc500
dc.subject.deweyCienciases_ES
dc.subject.ods09 Industry, innovation and infrastructure
dc.subject.odspa09 Industria, innovación e infraestructura
dc.titleBiofilm formation on collagen substrates modulates Streptococcus mutans bacterial extracellular nanovesicle production and cargo
dc.typeartículo
sipa.codpersvinculados1027766
sipa.codpersvinculados217256
sipa.codpersvinculados1028068
sipa.codpersvinculados17164
sipa.codpersvinculados1024156
sipa.codpersvinculados1062336
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