LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA

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dc.contributor.authorPavon-Perez, Jessy
dc.contributor.authorOviedo, Claudia A.
dc.contributor.authorElso-Freudenberg, Mhartyn
dc.contributor.authorHenriquez-Aedo, Karem
dc.contributor.authorAranda, Mario
dc.date.accessioned2025-01-23T19:58:30Z
dc.date.available2025-01-23T19:58:30Z
dc.date.issued2019
dc.description.abstractA rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a chromatographic separation on a Kinetex XB Core-Shell C-18 (100 mm x 4.6 mm, 2.6 mu m) column, using a mixture of ultrapure pure water (A) with 0.5 % (v/v) formic acid and methanol (B) as mobile phase. Analysis of L-DOPA was carried out by MS/MS applying a Multiple Reaction Monitoring (MRM) method using the transition m/z 198 -> m/z 152. This LC-MS/MS method allowed a well-resolved detection of L-DOPA in ca. 2 min within 6 min run. Method was validated showing a linear range from 0.05 to 10 mg L-1 (R-2 = 0.99); repeatability showed RSD value of 1.40%. Recoveries ranged from 94.14 to 116.62% with RSD values <= 5.66% and detection and quantification limits were 0.01 and 0.05 mg L-1, respectively. Applying this validated method, L-DOPA was determined in Vicia faba samples to determined its tissue distribution. As expected, a broad range of L-DOPA content finding the lowest content in seeds (4.72 +/- 2.12 mg g(-1)) and the highest in sprouts (133.60 +/- 5.65 mg g(-1)).
dc.fuente.origenWOS
dc.identifier.issn0717-9707
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100759
dc.identifier.wosidWOS:000509863600014
dc.issue.numero4
dc.language.isoen
dc.pagina.final4653
dc.pagina.inicio4651
dc.revistaJournal of the chilean chemical society
dc.rightsacceso restringido
dc.subjectMass spectrometry
dc.subjectL-DOPA
dc.subjectVicia faba
dc.subjectLC-MS/MS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
dc.typeartículo
dc.volumen64
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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