Neurotoxicity of acetylcholinesterase amyloid β-peptide aggregates is dependent on the type of Aβ peptide and the AChE concentration present in the complexes

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dc.contributor.authorMuñoz, FJ
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:32:04Z
dc.date.available2025-01-21T01:32:04Z
dc.date.issued1999
dc.description.abstractAlzheimer's disease (AD) is a neurodegenerative disorder whose hallmark is the presence of senile plaques and neurofibrillary tangles, Senile plaques are mainly composed of amyloid beta-peptide (A beta) fibrils and several proteins including acetylcholinesterase (AChE), AChE has been previously shown to stimulate the aggregation of A beta(1-40) into amyloid fibrils, In the present work, the neurotoxicity of different amyloid aggregates formed in the absence or presence of AChE was evaluated in rat pheochromocytoma PC12 cells. Stable AChE-A beta complexes were found to be more toxic than those formed without the enzyme, for A beta(1-40) and A beta(1-42), but not for amyloid fibrils formed with A beta(Val18-->Ala), a synthetic variant of the A beta(1-40) peptide. Of all the AChE-A beta complexes tested the one containing the A beta(1-40) peptide was the most toxic, When increasing concentrations of AChE were used to aggregate the A beta(1-40) peptide, the neurotoxicity of the complexes increased as a function of the amount of enzyme bound to each complex. Our results shea that AChE-A beta(1-40) aggregates are more toxic than those of AChE-A beta(1-42) and that the neurotoxicity depends on the amount of AChE bound to the complexes, suggesting that AChE may play a key role in the neurodegeneration observed in Alzheimer brain. (C) 1999 Federation of European Biochemical Societies.
dc.fuente.origenWOS
dc.identifier.issn0014-5793
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97199
dc.identifier.wosidWOS:000080333000007
dc.issue.numero3
dc.language.isoen
dc.pagina.final209
dc.pagina.inicio205
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectAlzheimer's disease
dc.subjectamyloid beta(1-40)-peptide
dc.subjectamyloid beta(1-42)-peptide
dc.subjectacetylcholinesterase
dc.subjectneurotoxicity
dc.subjectPC12 cell
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNeurotoxicity of acetylcholinesterase amyloid β-peptide aggregates is dependent on the type of Aβ peptide and the AChE concentration present in the complexes
dc.typeartículo
dc.volumen450
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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