CREATINE-KINASE PROTEIN-SEQUENCE ENCODED BY A CDNA MADE FROM TORPEDO-CALIFORNICA ELECTRIC ORGAN MESSENGER-RNA

Simple item page
dc.contributor.authorWEST, BL
dc.contributor.authorBABBITT, PC
dc.contributor.authorMENDEZ, B
dc.contributor.authorBAXTER, JD
dc.date.accessioned2025-01-23T19:43:50Z
dc.date.available2025-01-23T19:43:50Z
dc.date.issued1984
dc.description.abstractCreatine kinase (ATP creatine N-phosphotransferase, EC 2.7.3.2) is important in the maintenance of ATP levels in high energy-requiring tissues such as muscle and brain. A complete understanding of its function requires knowledge of its amino acid sequence. To obtain cDNA [complementary DNA] clones encoding creatine kinase sequences, a cDNA bank was constructed using mRNA from the electric organ of T. californica and was screened by comparing differential colony hybridization of electric organ and liver-derived 32P-labeled cDNA. Cloned DNA were isolated that can arrest the abundant synthesis of MW 40,000-43,000 material seen after in vitro translation of electric organ mRNA. One of the clones, CK52g8, was sequenced by the dideoxy M13 method and was found to encode a MW 42,941 protein, which is 68% homologous to a known partial sequence of rabbit muscle creatine kinases and which has a composition similar to creatine kinases from chicken and rabbit tissues. By contrast, no significant homology was found with the known sequences of kinases that use other substrates. RNA blot hybridization analysis indicated that CK52g8 is complementary to a 1600-base-pair mRNA. Primer extension analysis indicated that CK52g8 is only 5 nucleotides short of a full-length cDNA, implying that it encodes a complete protein sequence. The availability of this complete sequence should be useful in further studies of creatine kinase structure and function using techniques such as site-specific mutagenesis.
dc.fuente.origenWOS
dc.identifier.issn0027-8424
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99835
dc.identifier.wosidWOS:A1984TU17200021
dc.issue.numero22
dc.language.isoen
dc.pagina.final7011
dc.pagina.inicio7007
dc.revistaProceedings of the national academy of sciences of the united states of america-biological sciences
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCREATINE-KINASE PROTEIN-SEQUENCE ENCODED BY A CDNA MADE FROM TORPEDO-CALIFORNICA ELECTRIC ORGAN MESSENGER-RNA
dc.typeartículo
dc.volumen81
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas