The polarized localization of lipoprotein receptors and cholesterol transporters in the syncytiotrophoblast of the placenta is reproducible in a monolayer of primary human trophoblasts

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dc.contributor.authorFuenzalida, Barbara
dc.contributor.authorKallol, Sampada
dc.contributor.authorLuthi, Michael
dc.contributor.authorAlbrecht, Christiane
dc.contributor.authorLeiva, Andrea
dc.date.accessioned2025-01-20T23:52:24Z
dc.date.available2025-01-20T23:52:24Z
dc.date.issued2021
dc.description.abstractIntroduction: The uptake of low- and high-density lipoproteins (LDL and HDL) through the LDL receptor (LDLR) and the scavenger receptor class B type I (SR-BI) mediates maternal to fetal cholesterol transfer in syncytiotrophoblast (STB) cells. STB cells deliver cholesterol via cholesterol efflux through the ATP-binding cassette transporters A1 (ABCA1, to ApoA-I), G1 (ABCG1, to HDL), and SR-BI (to HDL). In the human placenta, these proteins are localized in the apical (LDLR, SR-BI, ABCA1) and basal (SR-BI, ABCA1, ABCG1) membrane of STB cells. However, whether these proteins in polarized primary culture models of STB show a similar localization to those in the human placenta is currently unknown.
dc.description.abstractMethods: Primary human trophoblasts (PHT) were isolated from normal placentas and cultured in Transwells (R) with Matrigel to obtain a polarized STB monolayer, proteins were determined by immunofluorescence and cholesterol efflux determined to different acceptors.
dc.description.abstractResults: At day 5, LDLR and ABCA1 localized mainly in the apical membrane, ABCG1 in the basal membrane, and SR-BI in both. Cholesterol efflux towards the apical compartment was higher to adult and neonatal HDL compared to ApoA-I. When acceptors were added in the basal compartment, cholesterol was retained in the Matrigel.
dc.description.abstractDiscussion: Polarized STB monolayers express LDLR, SR-BI, ABCA1 and ABCG1, and their apical/basal localization resembles the one described in human placental tissue. This study confirms the high physiological value and suitability of this model for use in functional studies. Our findings also suggest that ABCA1 and SR-BI participate in cholesterol efflux to the maternal side of the cells.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.placenta.2021.01.019
dc.identifier.eissn1532-3102
dc.identifier.issn0143-4004
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2021.01.019
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94912
dc.identifier.wosidWOS:000623753500007
dc.language.isoen
dc.pagina.final60
dc.pagina.inicio50
dc.revistaPlacenta
dc.rightsacceso restringido
dc.subjectCholesterol transfer
dc.subjectCholesterol transporters
dc.subjectLipoprotein receptors
dc.subjectPolarized trophoblast monolayer
dc.subjectCholesterol efflux
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe polarized localization of lipoprotein receptors and cholesterol transporters in the syncytiotrophoblast of the placenta is reproducible in a monolayer of primary human trophoblasts
dc.typeartículo
dc.volumen105
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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