Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements <i>in trans</i> a TMV-U1 trafficking-deficient mutant

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dc.contributor.authorDiaz-Griffero, Felipe
dc.contributor.authorCancino, Carmen Espinoza
dc.contributor.authorArevalo, Consuelo Medina
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-21T01:06:30Z
dc.date.available2025-01-21T01:06:30Z
dc.date.issued2006
dc.description.abstractTobamovirus movement proteins play a determinant role in the establishment of infections in plants. allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Q function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection,. local necrotic lesions were developed in resistant transgenic plants. and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants. causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg. even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96169
dc.identifier.wosidWOS:000238917800009
dc.issue.numero2
dc.language.isoen
dc.pagina.final279
dc.pagina.inicio269
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectmovement protein
dc.subjectcrucifer-infecting tobamovirus
dc.subjectviral complementation
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleExpression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements <i>in trans</i> a TMV-U1 trafficking-deficient mutant
dc.typeartículo
dc.volumen39
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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