Differential localization of α' and β subunits of protein kinase CK2 during rat spermatogenesis

Simple item page
dc.contributor.authorAlvarado-Diaz, Carlos P.
dc.contributor.authorTapia, Julio C.
dc.contributor.authorAntonelli, Marcelo
dc.contributor.authorMoreno, Ricardo D.
dc.date.accessioned2025-01-21T00:07:57Z
dc.date.available2025-01-21T00:07:57Z
dc.date.issued2009
dc.description.abstractProtein kinase CK2 is a serine/threonine kinase expressed in organisms from yeast to human and is composed of a catalytic subunit (alpha or alpha') and a regulatory subunit (beta) forming a holoenzyme with the possible subunit combinations alpha(2)beta(2), alpha'(2)beta(2), or alpha alpha'beta(2). This kinase has been shown to be involved in embryonic development and gametogenesis. We have studied the expression of the CK2 alpha' and CK2 beta subunits during the first wave of spermatogenesis and in adult testis in the rat. Western blot analyses have demonstrated that both CK2 alpha' and CK2 beta are expressed in testes from birth to adulthood. A more detailed study of the protein localization of CK2 alpha' and CK2 beta by immunohistochemistry suggests that CK2 alpha' and CK2 beta are localized in the nuclei of Sertoli cells in 5-day-old rats, whereas they appear to have a cytoplasmic localization in older animals. In adult testes, CK2 alpha' and CK2 beta subunits are present in spermatocytes. Both subunits exhibit a similar expression pattern with the highest level in spermatocytes at stages VIII-XIV. Interestingly, CK2 beta is highly expressed in spermatogonia, whereas CK2 alpha' is barely detectable. Mature epididymal spermatozoa express CK2 alpha' in the acrosome and CK2 beta in the flagellum. This new evidence therefore indicates that protein kinase CK2 has a possible role at various stages during mammalian spermatogenesis, a process that involves proliferation, meiosis, apoptosis, and differentiation. CK2 might thus emerge as a new pivotal control enzyme at various levels in mammalian spermatogenesis.
dc.description.funderFONDECYT
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00441-009-0847-1
dc.identifier.eissn1432-0878
dc.identifier.issn0302-766X
dc.identifier.urihttps://doi.org/10.1007/s00441-009-0847-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95650
dc.identifier.wosidWOS:000270737500012
dc.issue.numero1
dc.language.isoen
dc.pagina.final149
dc.pagina.inicio139
dc.revistaCell and tissue research
dc.rightsacceso restringido
dc.subjectSpermatogenesis
dc.subjectCK2
dc.subjectCytoskeleton
dc.subjectCell cycle
dc.subjectMeiosis
dc.subjectRat (Sprague Dawley)
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential localization of α' and β subunits of protein kinase CK2 during rat spermatogenesis
dc.typeartículo
dc.volumen338
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas