ClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl<SUP>-</SUP> channels and volume regulation in HeLa cells and <i>Xenopus laevis</i> oocytes

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dc.contributor.authorHermoso, M
dc.contributor.authorSatterwhite, CM
dc.contributor.authorAndrade, YN
dc.contributor.authorHidalgo, J
dc.contributor.authorWilson, SM
dc.contributor.authorHorowitz, B
dc.contributor.authorHume, JR
dc.date.accessioned2025-01-21T01:10:28Z
dc.date.available2025-01-21T01:10:28Z
dc.date.issued2002
dc.description.abstractVolume-sensitive osmolyte and anion channels (VSOACs) are activated upon cell swelling in most vertebrate cells. Native VSOACs are believed to be a major pathway for regulatory volume decrease (RVD) through efflux of chloride and organic osmolytes. ClC-3 has been proposed to encode native VSOACs in Xenopus laevis oocytes and in some mammalian cells, including cardiac and vascular smooth muscle cells. The relationship between the ClC-3 chloride channel, the native volume-sensitive osmolyte and anion channel (VSOAC) currents, and cell volume regulation in HeLa cells and X. laevis oocytes was investigated using ClC-3 antisense. In situ hybridization in HeLa cells, semiquantitative and real-time PCR, and immunoblot studies in HeLa cells and X. laevis oocytes demonstrated the presence of ClC-3 mRNA and protein, respectively. Exposing both cell types to hypotonic solutions induced cell swelling and activated native VSOACs. Transient transfection of HeLa cells with ClC-3 antisense oligonucleotide or X. laevis oocytes injected with antisense cRNA abolished the native ClC-3 mRNA transcript and protein and significantly reduced the density of native VSOACs activated by hypotonically induced cell swelling. In addition, antisense against native ClC-3 significantly impaired the ability of HeLa cells and X. laevis oocytes to regulate their volume. These results suggest that ClC-3 is an important molecular component underlying VSOACs and the RVD process in HeLa cells and X. laevis oocytes.
dc.description.funderNCRR NIH HHS
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M205132200
dc.identifier.eissn1083-351X
dc.identifier.issn0021-9258
dc.identifier.urihttps://doi.org/10.1074/jbc.M205132200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96687
dc.identifier.wosidWOS:000178662500127
dc.issue.numero42
dc.language.isoen
dc.pagina.final40074
dc.pagina.inicio40066
dc.revistaJournal of biological chemistry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl<SUP>-</SUP> channels and volume regulation in HeLa cells and <i>Xenopus laevis</i> oocytes
dc.typeartículo
dc.volumen277
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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