ATP Induces NO Production in Hippocampal Neurons by P2X<sub>7</sub> Receptor Activation Independent of Glutamate Signaling
| dc.contributor.author | Francisco Codocedo, Juan | |
| dc.contributor.author | Alejandro Godoy, Juan | |
| dc.contributor.author | Ines Poblete, Maria | |
| dc.contributor.author | Inestrosa, Nibaldo C. | |
| dc.contributor.author | Pablo Huidobro-Toro, Juan | |
| dc.date.accessioned | 2025-01-24T00:04:11Z | |
| dc.date.available | 2025-01-24T00:04:11Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | To assess the putative role of adenosine triphosphate (ATP) upon nitric oxide (NO) production in the hippocampus, we used as a model both rat hippocampal slices and isolated hippocampal neurons in culture, lacking glial cells. In hippocampal slices, additions of exogenous ATP or 2'(3')-O-(4-Benzoylbenzoyl) ATP (Bz-ATP) elicited concentration-dependent NO production, which increased linearly within the first 15 min and plateaued thereafter; agonist EC50 values were 50 and 15 mu M, respectively. The NO increase evoked by ATP was antagonized in a concentration-dependent manner by Coomassie brilliant blue G (BBG) or by N-omega-propyl-L-arginine, suggesting the involvement of P2X(7)Rs and neuronal NOS, respectively. The ATP induced NO production was independent of N-methyl-D-aspartic acid (NMDA) receptor activity as effects were not alleviated by DL-2-Amino-5-phosphonopentanoic acid (APV), but antagonized by BBG. In sum, exogenous ATP elicited NO production in hippocampal neurons independently of NMDA receptor activity. | |
| dc.fuente.origen | WOS | |
| dc.identifier.doi | 10.1371/journal.pone.0057626 | |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | https://doi.org/10.1371/journal.pone.0057626 | |
| dc.identifier.uri | https://repositorio.uc.cl/handle/11534/101795 | |
| dc.identifier.wosid | WOS:000315637900032 | |
| dc.issue.numero | 3 | |
| dc.language.iso | en | |
| dc.revista | Plos one | |
| dc.rights | acceso restringido | |
| dc.subject.ods | 03 Good Health and Well-being | |
| dc.subject.odspa | 03 Salud y bienestar | |
| dc.title | ATP Induces NO Production in Hippocampal Neurons by P2X<sub>7</sub> Receptor Activation Independent of Glutamate Signaling | |
| dc.type | artículo | |
| dc.volumen | 8 | |
| sipa.index | WOS | |
| sipa.trazabilidad | WOS;2025-01-12 |