ELECTRON-MICROSCOPIC MAPPING OF THERMUS-THERMOPHILUS RNA-POLYMERASE BINDING-SITES ON PLASMID PBR322

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dc.contributor.authorGONZALEZ, B
dc.contributor.authorDAVAGNINO, J
dc.contributor.authorVICUNA, R
dc.date.accessioned2025-01-23T19:27:34Z
dc.date.available2025-01-23T19:27:34Z
dc.date.issued1985
dc.description.abstractThe binding of RNA polymerase from the extreme thermophile T. thermophilus HB8 to plasmid pBR322 was measured by EM. DNA-protein complexes were prepared at 35 and 60.degree. C. At both temperatures the enzyme binds strongly to sites which coincide with promoters P1, P2, P3 and P4 present in pBR322. At 60.degree. C, an additional binding site appears, which is located between P3 and P4. There is a high degree of correlation between RNA polymerase binding sites and the location of A-T rich regions on pBR322 DNA.
dc.fuente.origenWOS
dc.identifier.eissn1879-1107
dc.identifier.issn1096-4959
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99740
dc.identifier.wosidWOS:A1985AEW7800016
dc.issue.numero3
dc.language.isoen
dc.pagina.final487
dc.pagina.inicio485
dc.revistaComparative biochemistry and physiology b-biochemistry & molecular biology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleELECTRON-MICROSCOPIC MAPPING OF THERMUS-THERMOPHILUS RNA-POLYMERASE BINDING-SITES ON PLASMID PBR322
dc.typeartículo
dc.volumen80
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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