Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture

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dc.contributor.authorVega, Andrea
dc.contributor.authorFredes, Isabel
dc.contributor.authorO'Brien, Jose
dc.contributor.authorShen, Zhouxin
dc.contributor.authorotvos, Krisztina
dc.contributor.authorAbualia, Rashed
dc.contributor.authorBenkova, Eva
dc.contributor.authorBriggs, Steven P.
dc.contributor.authorGutierrez, Rodrigo A.
dc.date.accessioned2025-01-20T22:12:08Z
dc.date.available2025-01-20T22:12:08Z
dc.date.issued2021
dc.description.abstractNitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate.
dc.fuente.origenWOS
dc.identifier.doi10.15252/embr.202051813
dc.identifier.eissn1469-3178
dc.identifier.issn1469-221X
dc.identifier.urihttps://doi.org/10.15252/embr.202051813
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94425
dc.identifier.wosidWOS:000681754200001
dc.issue.numero9
dc.language.isoen
dc.revistaEmbo reports
dc.rightsacceso restringido
dc.subjectauxin
dc.subjectHPLC-MS/MS
dc.subjectnitrate signaling
dc.subjectnitrogen response
dc.subjectphosphorylation
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleNitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture
dc.typeartículo
dc.volumen22
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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