The prolactin inhibition of follicle-stimulating hormone-induced aromatase activity in cultured rat granulosa cells is in part tyrosine kinase and protein kinase-C dependent

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dc.contributor.authorAlberto Villanueva, Luis
dc.contributor.authorMendez, Isabel
dc.contributor.authorAmpuero, Sandra
dc.contributor.authorLarrea, Fernando
dc.date.accessioned2025-01-21T01:33:46Z
dc.date.available2025-01-21T01:33:46Z
dc.date.issued1996
dc.description.abstractThe inhibitory actions of prolactin on gonadal steroidogenesis have been reported in different species and under a variety of experimental approaches. In this study, the mechanisms of the in-vitro effects of human prolactin (hPRL) on human follicle stimulating hormone (hFSH)-induced aromatase activity were determined using cultured granulosa cells from diethylstilboestrol (DES)-primed immature rats. Human PRL caused a dose-dependent decrease in hFSH-induced 17 beta-oestradiol production, even when cells were cultured in the presence of a cAMP analogue (8-Br-cAMP). These effects of hPRL appeared to be specific, since addition of an anti-rat PRL receptor monoclonal antibody (mAb) mimicked the hPRL inhibitory effect upon steroidogenesis in rat granulosa cells. In order to assess the importance of tyrosine kinase and protein kinase-C activation in the hPRL inhibitory effects upon oestrogen biosynthesis, cells were cultured in the presence of kinase inhibitors. The results showed that addition of genistein or staurosporine (a tyrosine kinase and protein kinase-C antagonist respectively) to cultured granulosa cells resulted in potent inhibition of hPRL actions upon hFSH-induced aromatization in a dose-dependent manner. These observations suggest that tyrosine kinase and protein kinase-C activation are involved in the biochemical events leading to hPRL inhibitory effects at the gonadal level.
dc.fuente.origenWOS
dc.identifier.doi10.1093/molehr/2.10.725
dc.identifier.eissn1460-2407
dc.identifier.urihttps://doi.org/10.1093/molehr/2.10.725
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97467
dc.identifier.wosidWOS:000560596700002
dc.issue.numero10
dc.language.isoen
dc.pagina.final731
dc.pagina.inicio725
dc.revistaMolecular human reproduction
dc.rightsacceso restringido
dc.subjectovary
dc.subjectprolactin
dc.subjectprotein kinases
dc.subjectsteroidogenesis
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe prolactin inhibition of follicle-stimulating hormone-induced aromatase activity in cultured rat granulosa cells is in part tyrosine kinase and protein kinase-C dependent
dc.typeartículo
dc.volumen2
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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