Hyperventilation worsens inflammatory lung injury in spontaneously breathing rats

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dc.article.numbere20240269
dc.catalogadorgjm
dc.contributor.authorFerreira, Juliana Dias Nascimento
dc.contributor.authorReboredo, Maycon Moura
dc.contributor.authorCosta, Eduardo Leite Vieira
dc.contributor.authorda Fonseca, Lidia Maria Carneiro
dc.contributor.authorRetamal Montes, Jaime
dc.contributor.authorSantos, Fabricio Junio Mendes
dc.contributor.authorde Paoli, Flavia
dc.contributor.authorda Fonseca, Adenilson de Souza
dc.contributor.authorLucinda, Leda Marilia Fonseca
dc.contributor.authorPinheiro, Bruno Valle
dc.date.accessioned2025-03-05T18:56:58Z
dc.date.available2025-03-05T18:56:58Z
dc.date.issued2024
dc.description.abstractObjectives: Here, we investigated the effects of hyperventilation on acute lung injury (ALI) in spontaneously breathing rats. Methods: Wistar rats were randomized to receive either intraperitoneal lipopolysaccharides (LPS) or saline, and intravenous infusion of NH4Cl (to induce metabolic acidosis and hyperventilation) or saline. Four groups were established: control-control (C-C), control-hyperventilation (C-HV), LPS-control (LPS-C), and LPS-hyperventilation (LPS-HV). Venous blood gases were collected before and after NH4Cl infusion and analyzed to confirm the presence of metabolic acidosis and hyperventilation. After euthanasia, lung injury was assessed using the ALI score, morphometric quantification of perivascular edema, neutrophil counts in the bronchoalveolar lavage, and mRNA expression of biological markers in the lung tissue. Results: Hyperventilation induced inflammatory lung injury in previously healthy lungs and exacerbated injuries previously induced by LPS (ALI score: C-C=0.14 [IQR 0.12; 0.56; 0.62]; p<0.01). Perivascular edema, neutrophil counts in bronchoalveolar lavage, and amphiregulin mRNA expression were higher in the LPS-HV group compared to the control group. Conclusions: Hyperventilation increased inflammatory injury in rats with ALI during spontaneous ventilation. These results suggest that the impact of vigorous spontaneous breathing efforts on worsening inflammatory lung injury warrants further investigation.
dc.description.funderNational Council for Scientific and Technological Development (CNPq)
dc.fechaingreso.objetodigital2025-03-05
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.36416/1806-3756/e20240269
dc.identifier.eissn1806-3756
dc.identifier.issn1806-3713
dc.identifier.urihttps://doi.org/10.36416/1806-3756/e20240269
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102378
dc.identifier.wosidWOS:001366159500002
dc.information.autorucEscuela de Medicina; Retamal Montes, Jaime; 0000-0002-6817-3659; 175147
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaJornal Brasileiro de Pneumologia
dc.rightsacceso abierto
dc.rights.licenseCC BY-NC 4.0 Attribution-NonCommercial 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAcute lung injury
dc.subjectAcute respiratory distress syndrome
dc.subjectSpontaneous breathing
dc.subjectHyperventilation
dc.subjectLung injury
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHyperventilation worsens inflammatory lung injury in spontaneously breathing rats
dc.typeartículo
dc.volumen50
sipa.codpersvinculados175147
sipa.trazabilidadWOS;2024-12-14
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