Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES

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dc.contributor.authorConejeros-Lillo, Sabrina
dc.contributor.authorAguirre, Francisco
dc.contributor.authorCabrera, Daniel
dc.contributor.authorSimon, Felipe
dc.contributor.authorPenailillo, Luis
dc.contributor.authorCabello-Verrugio, Claudio
dc.date.accessioned2025-01-20T17:06:42Z
dc.date.available2025-01-20T17:06:42Z
dc.date.issued2024
dc.description.abstractSarcopenia is characterized by reduced muscle strength and mass, and a decline in muscle fiber diameter and amount of sarcomeric proteins. Sarcopenia involves the activation of the ubiquitinproteasome system (UPS). MuRF-1 and atrogin-1 are E3 ubiquitin ligases belonging to UPS, leading to proteolysis mediated by the PSMB 5, 6, and 7 subunits of 20S proteasome. CCL5/RANTES induces a sarcopenic-like effect in muscle cells. The present work explored the impact of CCL5 on UPS components and the influence of UPS on its sarcopenic-like effect. We demonstrated that CCL5 increased MuRF-1 and atrogin-1 protein levels and mRNA levels of subunits PSMB 5, 6, and 7. We used the MG132 inhibitor to elucidate the role of the 20S proteasome in the CCL5-induced sarcopenic-like effect. This inhibitor prevented the decrease in troponin and MHC protein levels and partially stopped the reduction in the diameter of singleisolated flexor digitorum brevis (FDB) muscle fibers induced by CCL5. These findings indicate that CCL5 actively modulates the UPS. Moreover, our results show the direct participation of UPS in the sarcopenic-like phenotype induced by CCL5.
dc.fuente.origenWOS
dc.identifier.doi10.4081/ejtm.2024.12249
dc.identifier.eissn2037-7460
dc.identifier.issn2037-7452
dc.identifier.urihttps://doi.org/10.4081/ejtm.2024.12249
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90811
dc.identifier.wosidWOS:001194891100004
dc.issue.numero1
dc.language.isoen
dc.revistaEuropean journal of translational myology
dc.rightsacceso restringido
dc.subjectIrisin
dc.subjectsaliva
dc.subjectserum
dc.subjectmuscle
dc.subjectphysical exercise
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRole of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES
dc.typeartículo
dc.volumen34
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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