Porphyrin nanoemulsion for antimicrobial photodynamic therapy: effective delivery to inactivate biofilm-related infections

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dc.contributor.authorBuzza, Hilde Harb
dc.contributor.authorAlves, Fernanda
dc.contributor.authorTome, Ana Julia Barbosa
dc.contributor.authorChen, Juan
dc.contributor.authorKassab, Giulia
dc.contributor.authorBu, Jiachuan
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorZheng, Gang
dc.contributor.authorKurachi, Cristina
dc.date.accessioned2025-01-20T20:23:06Z
dc.date.available2025-01-20T20:23:06Z
dc.date.issued2022
dc.description.abstractThe management of biofilm-related infections is a challenge in healthcare, and antimicrobial photodynamic therapy (aPDT) is a powerful tool that has demonstrated a broad-spectrum activity. Nanotechnology has been used to increase the aPDT effectiveness by improving the photosensitizer's delivery properties. NewPS is a simple, versatile, and safe surfactant-free nanoemulsion with a porphyrin salt shell encapsulating a food-grade oil core with promising photodynamic action. This study evaluated the use of NewPS for aPDT against microorganisms in planktonic, biofilm, and in vivo models of infected wounds. First, the potential of NewPS-mediated aPDT to inactivate Streptococcus pneumoniae and Staphylococcus aureus suspensions was evaluated. Then, a series of protocols were assessed against S. aureus biofilms by means of cell viability and confocal microscopy. Finally, the best biofilm protocol was used for the treatment of S. aureus in a murine-infected wound model. A high NewPS-bacteria cell interaction was achieved since 0.5 nM and 30 J/cm(2) was able to kill S. pneumoniae suspension. In the S. aureus biofilm, enhanced efficacy of NewPS-aPDT was achieved when 100 mu M of NewPS was applied with longer periods of incubation at the light dose of 60 J/cm(2). The best single and double-session protocol reduced 5.56 logs and 6.03 logs, respectively, homogeneous NewPS distribution, resulting in a high number of dead cells after aPDT. The in vivo model showed that one aPDT session enabled a reduction of 6 logs and faster tissue healing than the other groups. In conclusion, NewPS-aPDT may be considered a safe and effective anti-biofilm antimicrobial photosensitizer.
dc.fuente.origenWOS
dc.identifier.doi10.1073/pnas.2216239119
dc.identifier.eissn1091-6490
dc.identifier.issn0027-8424
dc.identifier.urihttps://doi.org/10.1073/pnas.2216239119
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92710
dc.identifier.wosidWOS:000980665600015
dc.issue.numero46
dc.language.isoen
dc.revistaProceedings of the national academy of sciences of the united states of america
dc.rightsacceso restringido
dc.subjectnanoemulsion
dc.subjectantimicrobial photodynamic therapy
dc.subjectbiofilm
dc.subjectStaphylococcus aureus
dc.subjectStreptococcus pneumoniae
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePorphyrin nanoemulsion for antimicrobial photodynamic therapy: effective delivery to inactivate biofilm-related infections
dc.typeartículo
dc.volumen119
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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