Multivariate optimization of microwave-assisted enzyme digestion of α-casein for generation of bioactive peptides

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dc.contributor.authorLopez, Katherine
dc.contributor.authorEspinoza-Bello, Alejandra
dc.contributor.authorCarrasco, Jonathan
dc.contributor.authorPena-Farfal, Carlos
dc.contributor.authorAranda, Mario
dc.contributor.authorHenriquez-Aedo, Karem
dc.date.accessioned2025-01-20T17:14:09Z
dc.date.available2025-01-20T17:14:09Z
dc.date.issued2023
dc.description.abstractCaseins are the principal milk proteins and serve as an important source of bioactive peptides with diverse beneficial effects for human health, such as antihypertensive, immunomodulating, anti-inflammatory, and antithrombotic. The objective of this study was to optimize, using the design of the experiment, the production of bioactive peptides from alpha-casein applying microwave -assisted enzyme digestion (MAED). The optimal MAED conditions (time, temperature, and enzyme/protein ratio) were established for pepsin (digestion time 4 min, temperature 41 degrees C, and E:P ratio 1:40) and trypsin (digestion time 10 min, temperature 37 degrees C and E:P ratio 1:200.) enzymes. Digestion yields and the intensity of different bioactivities, i.e., antimicrobial, antioxidant, and acetylcholinesterase and alpha glucosidase inhibitory activities, evaluated by the HPTLC-bioassay technique were used as response variables. Trypsin-formed alpha-casein peptides showed antimicrobial, antioxidant, and acetylcholinesterase inhibition activities, while pepsin -formed showed antimicrobial, antioxidant, and alpha-glucosidase inhibition activities. These results demonstrate that MAED is a fast and effective technique for bioactive peptide production from casein proteins.
dc.fuente.origenWOS
dc.identifier.issn0717-9707
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91279
dc.identifier.wosidWOS:001210065500006
dc.issue.numero3
dc.language.isoen
dc.pagina.final5968
dc.pagina.inicio5963
dc.revistaJournal of the chilean chemical society
dc.rightsacceso restringido
dc.subjectBioactive peptides
dc.subjectalpha-casein
dc.subjectEDA-HPTLC-bioassay
dc.subjectalpha-glucosidase
dc.subjectacetylcholinesterase
dc.subjectantioxidant
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMultivariate optimization of microwave-assisted enzyme digestion of α-casein for generation of bioactive peptides
dc.typeartículo
dc.volumen68
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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