Bacterial-derived extracellular polysaccharides reduce antimicrobial susceptibility on biotic and abiotic surfaces

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dc.contributor.authorSouza, Joao Gabriel S.
dc.contributor.authorOliveira, Barbara E. Costa
dc.contributor.authorCosta, Raphael C.
dc.contributor.authorBechara, Karen
dc.contributor.authorCardoso-Filho, Otavio
dc.contributor.authorBenso, Bruna
dc.contributor.authorShibli, Jamil Awad
dc.contributor.authorBertolini, Martinna
dc.contributor.authorBarao, Valentim A. R.
dc.date.accessioned2025-01-20T21:02:51Z
dc.date.available2025-01-20T21:02:51Z
dc.date.issued2022
dc.description.abstractObjective: Extracellular biofilm matrix plays a role in reducing bacterial susceptibility against antimicrobials. Since the surface where biofilm is growing modulates microbial accumulation and bacterial-derived exopoly-saccharides (EPS) synthesis, this study compared the role of EPS to reduce antimicrobial susceptibility on biotic (dental surface) and abiotic (titanium (Ti) material) surfaces and the effect of remaining matrix-enriched biofilms to promote bacterial recolonization. Design: 48 h Streptococcus mutans UA159 strain biofilms were grown on enamel and Ti surfaces. The medium was supplemented with 1% sucrose, substrate for EPS synthesis, or with 0.5% glucose + 0.5% fructose as control. Chlorhexidine (CHX) 0.2% was used for antimicrobial treatment. Biofilms were collected and the following analyses were considered: viable bacterial counts, biofilm pH, EPS content, and biofilm structure by scanning electron microscopy and confocal laser scanning microscopy (CLSM). Substrate surfaces were analyzed by 3D laser scanning confocal microscope. Results: Enamel surface showed a higher amount of EPS content (p < 0.05), which may be explained by the higher bacterial biomass compared to Ti material. EPS content reduced bacterial susceptibility against antimi-crobial treatments for both substrates, compared to EPS control (p < 0.05). However, sucrose-treated cells presented the same magnitude of reduction for Ti or enamel. Interestingly, matrix-enriched biofilms favored bacterial recolonization for both substrates. Conclusion: The surface where the biofilm is growing modulates the amount of EPS synthesized and matrix content plays a key role in reducing antimicrobial susceptibility and promoting bacterial recolonization.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.archoralbio.2022.105521
dc.identifier.eissn1879-1506
dc.identifier.issn0003-9969
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2022.105521
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93092
dc.identifier.wosidWOS:000848589300002
dc.language.isoen
dc.revistaArchives of oral biology
dc.rightsacceso restringido
dc.subjectTitanium
dc.subjectDental enamel
dc.subjectBiofilms
dc.subjectBiofilm matrix
dc.subjectChlorhexidine
dc.subjectAntimicrobial agents
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBacterial-derived extracellular polysaccharides reduce antimicrobial susceptibility on biotic and abiotic surfaces
dc.typeartículo
dc.volumen142
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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