Global Methylation Analysis Using MSAP Reveals Differences in Chilling-Associated DNA Methylation Changes during Dormancy Release in Contrasting Sweet Cherry Varieties

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dc.contributor.authorNarvaez, Gabriela
dc.contributor.authorMunoz-Espinoza, Claudia
dc.contributor.authorSoto, Esteban
dc.contributor.authorRothkegel, Karin
dc.contributor.authorBastias, Macarena
dc.contributor.authorGutierrez, Jose
dc.contributor.authorBravo, Soraya
dc.contributor.authorHasbun, Rodrigo
dc.contributor.authorMeneses, Claudio
dc.contributor.authorMiyasaka Almeida, Andrea
dc.date.accessioned2025-01-20T21:01:44Z
dc.date.available2025-01-20T21:01:44Z
dc.date.issued2022
dc.description.abstractDormancy is an adaptive strategy developed by temperate perennial crops to protect overwinter tissues from unfavorable environmental conditions. Sweet cherry (Prunus avium L.), a member of the Rosaceae family, requires chilling to overcome dormancy. The time of harvest is directly correlated with chilling requirements in sweet cherries. Consequently, early and late season varieties have low and high chilling requirements, respectively. There is evidence that the expression of dormancy-related genes is regulated by DNA methylation. In this work, methylation-sensitive amplified polymorphism (MSAP) was applied to study genome-wide DNA methylation changes associated with dormancy in two low-chill varieties, 'Royal Dawn' and 'Glen Red', and one high-chill variety, 'Kordia'. Our primary results suggest that the occurrence of progressive DNA demethylation is associated with chilling accumulation during dormancy in the three varieties, independent of their chilling requirements. Genes were identified with different methylation status changes, detected by MSAP, related to cell wall remodeling and energy metabolism. Several MSAP profiles among the varieties were observed, suggesting that fine epigenetic control is required to coordinate hormonal and environmental signals that induce dormancy and its release.
dc.fuente.origenWOS
dc.identifier.doi10.3390/horticulturae8100962
dc.identifier.eissn2311-7524
dc.identifier.urihttps://doi.org/10.3390/horticulturae8100962
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92943
dc.identifier.wosidWOS:000872866800001
dc.issue.numero10
dc.language.isoen
dc.revistaHorticulturae
dc.rightsacceso restringido
dc.subjectPrunus avium
dc.subjectchilling requirement
dc.subjectdormancy
dc.subjectDNA methylation
dc.subjectepigenetics
dc.subjectepialleles
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGlobal Methylation Analysis Using MSAP Reveals Differences in Chilling-Associated DNA Methylation Changes during Dormancy Release in Contrasting Sweet Cherry Varieties
dc.typeartículo
dc.volumen8
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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