<i>Sirex</i><i> noctilio</i> infestation led to inevitable pine death despite activating pathways involved in tolerance

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dc.contributor.authorRiquelme, Sebastian
dc.contributor.authorCampos, Jasna, V
dc.contributor.authorPecio, Lukasz
dc.contributor.authorAlzamora, Rosa
dc.contributor.authorMardones, Claudia
dc.contributor.authorSimonet, Ana M.
dc.contributor.authorArteaga-Perez, Luis E.
dc.contributor.authorRubilar, Rafael
dc.contributor.authorFiehn, Oliver
dc.contributor.authorPerez, Andy J.
dc.date.accessioned2025-01-20T21:02:51Z
dc.date.available2025-01-20T21:02:51Z
dc.date.issued2022
dc.description.abstractDefense-related metabolome traits in pine species after infestation by Sirex noctilio are largely unknown, despite, in most cases, trees being overwhelmed. Using LC-MS-based untargeted metabolomics, we revealed the systemic metabolic changes induced by this insect in 14-year-old Pinus radiata trees, the most affected species worldwide. An immediate metabolome alteration was expressed in needles after infestation, including the up-regulation of flavonols, flavan-3-ols, oxyneolignans, auxins, proline, and tryptophan, among others. The flavan-3-ols (catechin and procyanidin B1) suggested a rapidly induced photoprotection mechanism aided by diverting proline as an alternative substrate for respiration to compensate for the progressive chlorosis that degrades photosystems. Meanwhile, glutathione, glutamate, and ascorbate levels significantly dropped in needles, which may indicate the critical oxidative stress that trees had to face since the onset of the infestation. They were not fully replenished after long-term infestation, and redox homeostasis was probably not achieved, compromising tree survival. Nevertheless, a huge auxins overexpression detected in needles throughout the infestation may reflect tolerance against the premature senescence caused by the woodwasp venom. In contrast, the metabolome of wood tissues remained initially unchanged, although it seems to collapse after three months. Overall, the metabolomics strategy adopted in this work evidenced its usefulness in uncovering the fundamental roles of plants' chemical defense that govern interactions with specific stressors.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.phytochem.2022.113350
dc.identifier.eissn1873-3700
dc.identifier.issn0031-9422
dc.identifier.urihttps://doi.org/10.1016/j.phytochem.2022.113350
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93091
dc.identifier.wosidWOS:000849134400007
dc.language.isoen
dc.revistaPhytochemistry
dc.rightsacceso restringido
dc.subjectPinus radiata D
dc.subjectdon
dc.subjectPinaceae
dc.subjectRadiata pine
dc.subjectLC -MS metabolomics
dc.subjectPlant -insect interaction
dc.subjectPlant defense
dc.subjectSirex noctilio
dc.subjectWoodwasp
dc.subject.ods15 Life on Land
dc.subject.ods13 Climate Action
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.subject.odspa13 Acción por el clima
dc.title<i>Sirex</i><i> noctilio</i> infestation led to inevitable pine death despite activating pathways involved in tolerance
dc.typeartículo
dc.volumen203
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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