Method for lineage typing of epidemic Renibacterium salmoninarum in Chilean salmon farms

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dc.catalogadorjwg
dc.contributor.authorMora Salas, Patricia Del Rosario
dc.contributor.authorZapararte, Sebastián
dc.contributor.authorVillouta Wheeler, Pamela Alejandra
dc.contributor.authorAraya León, Henry
dc.contributor.authorAvendaño Herrera, Rubén
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorMardones Loyola, Fernando Otoniel
dc.date.accessioned2025-02-06T19:28:07Z
dc.date.available2025-02-06T19:28:07Z
dc.date.issued2023
dc.description.abstractRenibacterium salmoninarum (Rs) is the etiological agent of bacterial kidney disease (BKD), which significantly affects farmed and wild salmonids worldwide. Although the whole genome of Rs (~3.1 million nucleotides) is highly conserved, genomic epidemiology analyses have identified four sub-lineages from Chilean isolates. A total of 94 Rs genomes from the BIGSdb aquaculture database were aligned and compared using bioinformatics tools, identifying 2199 independent single-nucleotide polymorphisms (SNPs) spread along the genome. A detailed analysis of the distribution of the SNPs showed five local zones of a length in the range of 10–15 kbp that should be used to unambiguously identify a specific sub-lineage. Based on the Rs type strain DSM 20767T, we designed multiplex PCR primers that produce specific amplification products which were further sequenced by the Sanger method to obtain the genotype of the sub-lineage. For the genetic typing, we evaluated 27 Rs isolates recovered from BKD outbreaks from different fish species and regions of Chile. Based on the findings reported here, we propose the PCR approach as a valuable tool for the rapid and reliable studying of the relationships between Rs isolates and the different sub-lineages without requiring the sequencing of the entire genome.
dc.description.funderFONDECYT
dc.description.funderAgencia Nacional de Investigación y Desarrollo
dc.format.extent8 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1111/jfd.13761
dc.identifier.eissn1365-2761
dc.identifier.issn0140-7775
dc.identifier.scopusid2-s2.0-85147211794
dc.identifier.urihttps://doi.org/10.1111/jfd.13761
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102183
dc.identifier.wosidWOS:000922654500001
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final506
dc.pagina.inicio499
dc.revistaJournal of Fish Diseases
dc.rightsacceso restringido
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMethod for lineage typing of epidemic Renibacterium salmoninarum in Chilean salmon farms
dc.typeartículo
dc.volumen46
sipa.codpersvinculados82342
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