<i>Penicillium purpurogenum</i> produces a family 1 acetyl xylan esterase containing a carbohydrate-binding module

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dc.contributor.authorGordillo, Felipe
dc.contributor.authorCaputo, Valentina
dc.contributor.authorPeirano, Alessandra
dc.contributor.authorChavez, Renato
dc.contributor.authorVan Beeumen, Jozef
dc.contributor.authorVandenberghe, Isabel
dc.contributor.authorClaeyssens, Marc
dc.contributor.authorBull, Paulina
dc.contributor.authorRavanal, Maria Cristina
dc.contributor.authorEyzaguirre, Jaime
dc.date.accessioned2025-01-21T01:05:52Z
dc.date.available2025-01-21T01:05:52Z
dc.date.issued2006
dc.description.abstractAt least three acetyl xylan esterases (AXE I, II and III) are secreted by Penicillium purpurogenum. This publication describes more detailed work on AXE I and its gene. AXE I binds cellulose but not xylan; it is glycosylated and inactivated by phenylmethylsulphonyl fluoride, showing that it is a serine esterase. The axe1 gene presents an open reading frame of 1278 bp, including two introns of 68 and 61 bp; it codes for a signal peptide of 31 residues and a mature protein of 351 amino acids (molecular weight 36,693). AXE I has a modular structure: a catalytic module at the amino terminus belonging to family I of the carbohydrate esterases, a linker rich in serines and threonines, and a family 1 carboxy terminal carbohydrate binding module (CBM). The CBM is similar to that of AXE from Trichoderma reesei, (with a family 5 catalytic module) indicating that the genes for catalytic modules and CBMs have evolved separately, and that they have been linked by gene fusion. The promoter sequence of axe1 contains several putative sequences for binding of gene expression regulators also found in other family 1 esterase gene promoters. It is proposed that AXE I and II act in succession in xylan degradation; first, xylan is attacked by AXE I and other xylanases possessing CBMs (which facilitate binding to lignocellulose), followed by other enzymes acting mainly on soluble substrates. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.mycres.2006.07.003
dc.identifier.issn0953-7562
dc.identifier.urihttps://doi.org/10.1016/j.mycres.2006.07.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96036
dc.identifier.wosidWOS:000242428700001
dc.language.isoen
dc.pagina.final1139
dc.pagina.inicio1129
dc.revistaMycological research
dc.rightsacceso restringido
dc.subjectAXE genes
dc.subjectenzymes gene regulation
dc.subjectxylan biodegradation
dc.subject.ods07 Affordable and Clean Energy
dc.subject.ods12 Responsible Consumption and Production
dc.subject.odspa07 Energía asequible y no contaminante
dc.subject.odspa12 Producción y consumo responsable
dc.title<i>Penicillium purpurogenum</i> produces a family 1 acetyl xylan esterase containing a carbohydrate-binding module
dc.typeartículo
dc.volumen110
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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