Does the release of tritiated noradrenaline accurately reflect the release of endogenous noradrenaline from rat vas deferens nerve terminals?

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dc.contributor.authorBitran, M
dc.contributor.authorTapia, W
dc.date.accessioned2025-01-21T01:33:37Z
dc.date.available2025-01-21T01:33:37Z
dc.date.issued1997
dc.description.abstractTo determine whether the release of tritiated noradrenaline (NA) front the sympathetic nerve terminals of the rat vas deferens is art accurate reflection of the release of endogenous NA, we compared the electrically-evoked release of tritiated and endogenous NA front the prostatic sections of the vasa deferentia of male rats.
dc.description.abstractWe found that while the release of tritiated NA was completely dependent on the presence of calcium, the release of endogenous NA was not. The overflow of both, tritiated and endogenous NA, was virtually unaffected by blockade of the neuronal uptake mechanism by desipramine. In contrast, blockade of the extraneuronal uptake greatly increased the overflow of endogenous NA, while having no effect on the overflow of tritiated NA. Tritiated NA release, on the other hand, was sensitive to prejunctional regulation, while the release of endogenous NA was not. Increases in stimulus train duration induced a significant increase in the release of endogenous NA, but not in that of tritiated NA. In contrast, the later responded to lower stimulus train frequencies and reached a plateau at lower frequency values as compared to the endogenous NA release.
dc.description.abstractOur results indicate the existence of marked differences between the release of tritiated and endogenous NA. We conclude that: I) the assumption that tritiated NA release provides a good marker for endogenous NA release in the rat vas deferens seems unwarranted; 2) the use of endogenous NA to study the release process in the vas deferens requires a re-examination of the experimental conditions used in order to minimize possible artifacts that may obscure the study of neuronal release, 3) the choice between measuring the release of tritiated or endogenous NA must be evaluated for each tissue in particular, taking into account its cytoarchitecture, as well as the experimental conditions used.
dc.fuente.origenWOS
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97446
dc.identifier.wosidWOS:A1997YC74100004
dc.issue.numero3
dc.language.isoen
dc.pagina.final115
dc.pagina.inicio105
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectnoradrenaline release
dc.subjecttritiated noradrenaline
dc.subjectvas deferens
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDoes the release of tritiated noradrenaline accurately reflect the release of endogenous noradrenaline from rat vas deferens nerve terminals?
dc.typeartículo
dc.volumen30
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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